Serological and molecular survey of Anaplasma species infection in dogs with suspected tickborne disease in Portugal
| Autor: | J.S. Dumler, Fátima Bacellar, Maria Sofia Núncio, Nuno Alexandre, Rita de Sousa, Ana Sofia Santos |
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| Rok vydání: | 2009 |
| Předmět: |
DNA
Bacterial Male Anaplasma platys animal diseases Prevalence Fluorescent Antibody Technique Biology Polymerase Chain Reaction law.invention Serology Dogs law parasitic diseases Animals Seroprevalence Serologic Tests Dog Diseases Seroconversion Phylogeny Polymerase chain reaction DNA Primers Polymorphism Genetic Portugal General Veterinary Ehrlichiosis General Medicine bacterial infections and mycoses biology.organism_classification Antibodies Bacterial Virology Anaplasma phagocytophilum Tick-Borne Diseases Immunoglobulin G biology.protein bacteria Female Antibody Databases Nucleic Acid |
| Zdroj: | Veterinary Record. 164:168-171 |
| ISSN: | 0042-4900 |
| DOI: | 10.1136/vr.164.6.168 |
| Popis: | Fifty-five dogs with suspected tickborne disease were tested by immunofluorescence assay and PCR for Anaplasma phagocytophilum infection. Thirty (54.5 per cent) of the dogs were seropositive and five of them fulfilled the serological criteria for an active infection, with either seroconversion or a fourfold increase in antibody titres. Fragments of DNA of the expected size were detected by PCR in two seropositive and three seronegative dogs. However, direct amplicon sequencing failed to identify active A phagocytophilum infections, but revealed the presence of Anaplasma platys DNA in the PCR-positive animals. |
| Databáze: | OpenAIRE |
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