Staphylococcus aureus Fur Regulates the Expression of Virulence Factors That Contribute to the Pathogenesis of Pneumonia
| Autor: | Federico C. Beasley, Brian D. Corbin, Ahmed S. Attia, David B. Friedman, David E. Heinrichs, Paul M. Dunman, Eric P. Skaar, W. Hayes McDonald, M. Indriati Hood, Devin L. Stauff, Lisa J. Zimmerman, William J. Mason, Kelsi L. Anderson, Victor J. Torres |
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| Rok vydání: | 2010 |
| Předmět: |
inorganic chemicals
Staphylococcus aureus Proteome Virulence Factors Immunology Virulence Molecular Genomics medicine.disease_cause Microbiology Mass Spectrometry Gene Knockout Techniques Mice Bacterial Proteins Immunity Pneumonia Staphylococcal medicine Animals Electrophoresis Gel Two-Dimensional Pathogen Regulation of gene expression integumentary system biology Gene Expression Profiling Gene Expression Regulation Bacterial biology.organism_classification medicine.disease Mice Inbred C57BL Repressor Proteins Gene expression profiling Disease Models Animal Infectious Diseases bacteria Female Parasitology Pneumonia (non-human) Bacteria Chromatography Liquid |
| Zdroj: | Infection and Immunity. 78:1618-1628 |
| ISSN: | 1098-5522 0019-9567 |
| DOI: | 10.1128/iai.01423-09 |
| Popis: | The tremendous success of Staphylococcus aureus as a pathogen is due to the controlled expression of a diverse array of virulence factors. The effects of host environments on the expression of virulence factors and the mechanisms by which S. aureus adapts to colonize distinct host tissues are largely unknown. Vertebrates have evolved to sequester nutrient iron from invading bacteria, and iron availability is a signal that alerts pathogenic microorganisms when they enter the hostile host environment. Consistent with this, we report here that S. aureus senses alterations in the iron status via the ferric uptake regulator (Fur) and alters the abundance of a large number of virulence factors. These Fur-mediated changes protect S. aureus against killing by neutrophils, and Fur is required for full staphylococcal virulence in a murine model of infection. A potential mechanistic explanation for the impact of Fur on virulence is provided by the observation that Fur coordinates the reciprocal expression of cytolysins and a subset of immunomodulatory proteins. More specifically, S. aureus lacking fur exhibits decreased expression of immunomodulatory proteins and increased expression of cytolysins. These findings reveal that Fur is involved in initiating a regulatory program that organizes the expression of virulence factors during the pathogenesis of S. aureus pneumonia. |
| Databáze: | OpenAIRE |
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