Constitutive expression ofArabidopsis NPR1confers enhanced resistance to the early instars ofSpodoptera liturain transgenic tobacco
| Autor: | Koppolu Raja Rajesh Kumar, Pulugurtha Bharadwaja Kirti, Madhusudhan Budatha, Gargi Meur, Aparna Dutta Gupta, Tantravahi Srinivasan |
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| Rok vydání: | 2008 |
| Předmět: |
Physiology
Transgene Arabidopsis Spodoptera litura Plant Science Genetically modified crops Spodoptera Genes Plant Gene Expression Regulation Plant Tobacco Botany Gene expression Genetics Animals Protease Inhibitors RNA Messenger Plant Diseases biology Arabidopsis Proteins fungi Feeding Behavior Cell Biology General Medicine Plants Genetically Modified biology.organism_classification Molecular biology Immunity Innate Gastrointestinal Tract Plant Leaves Larva Biological Assay Heterologous expression Salicylic Acid Systemic acquired resistance |
| Zdroj: | Physiologia Plantarum. 133:765-775 |
| ISSN: | 1399-3054 0031-9317 |
| Popis: | In Arabidopsis, NPR1 (AtNPR1) regulates salicylic acid (SA)-mediated activation of PR genes at the onset of systemic acquired resistance. AtNPR1 also modulates SA-induced suppression of jasmonic acid-responsive gene expression, and npr1 mutants manifest enhanced herbivore resistance. We have raised stable transgenic tobacco lines, expressing AtNPR1 constitutively, which showed elevated expression of PR1 and PR2 genes upon SA treatment. Herbivore bioassays with a generalist polyphagous pest, Spodoptera litura, revealed that the transgenic lines exhibited enhanced resistance compared to the wild-type plants, particularly with respect to younger larval populations. Insect-mediated injury induced several protease inhibitors (PIs), more significantly a 40-kDa serine PI in all the tobacco lines, but the induction was higher in the transgenic plants. We show in this communication that heterologous expression of AtNPR1 provides enhanced resistance to early larval populations of the herbivore, Spodoptera in transgenic tobacco plants. |
| Databáze: | OpenAIRE |
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