Effect of TGF-b1 stimulation on the secretome of human adipose-derived mesenchymal stromal cells
| Autor: | Alejandro José Saldías, Tania Melina Rodriguez, Ricardo A Dewey, Jorge Velasco Zamora, Marcelo Irigo, Marcelo J. Perone |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Adult
Chemokine Transforming Growth Factor-B1 CIENCIAS MÉDICAS Y DE LA SALUD medicine.medical_treatment Adipose tissue Inflammation Mscs Biology Mesenchymal Stem Cell Transplantation Antibodies Biotecnología de la Salud Proinflammatory cytokine Transforming Growth Factor beta1 medicine Humans purl.org/becyt/ford/3.4 [https] Secretome Tecnologías que involucran la manipulación de células tejidos órganos o todo el organismo Mesenchymal Stromal Cells Mesenchymal stem cell Mesenchymal Stem Cells Cell Biology General Medicine Tissue-Specific Progenitor and Stem Cells Cell biology Cytokine Adipose Tissue Immunology biology.protein Cytokines Secretion of Cytokines And Chemokines Female purl.org/becyt/ford/3 [https] Chemokines medicine.symptom Fetal bovine serum Developmental Biology Homing (hematopoietic) |
| Zdroj: | CONICET Digital (CONICET) Consejo Nacional de Investigaciones Científicas y Técnicas instacron:CONICET |
| Popis: | Adipose tissue is an attractive source of mesenchymal stromal cells (MSCs) owing to the relative ease of obtaining large volumes with more MSC abundance compared with other sources. Increasing evidence supports the fact that trophic factors secreted by MSCs play a pivotal therapeutic role. Several strategies in regenerative medicine use MSCs, mainly exploiting their immunosuppressive effect and homing capacity to sites of damage. Transforming growth factor-b1 (TGF-b1) is a pleiotropic cytokine that, depending on the cell niche, can display either anti-inflammatory or proinflammatory effects. TGF-b1 expression increases in various tissues with damage, especially when accompanied by inflammation. Thus, we analyzed the effect of TGF-b1 on the secretion by adipose-derived mesenchymal stromal cells (ASCs) of a panel of 80 cytokines/chemokines using an antibody array. To avoid a possible effect of fetal bovine serum (FBS) on ASCs secretion, we performed our analysis by culturing cells in FBS-free conditions, only supplemented with 0.1% of bovine serum albumin. We report the cytokine profile secreted by ASCs. We also found that TGF-b1 exposure modulates 8 chemokines and 18 cytokines, including TGF-b1 and -b2, and other important cytokines involved in immunosuppression, allergic responses, and bone resorption. Fil: Rodríguez, Tania Melina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Instituto de Investigaciones Biotecnológicas - Instituto Tecnológico Chascomús. Instituto de Investigaciones Biotecnológicas (sede Chascomús); Argentina. Universidad Nacional de San Martín; Argentina Fil: Saldias. Alejandro. Hospital Italiano de Buenos Aires; Argentina Fil: Irigo, Marcelo. Hospital Italiano de Buenos Aires; Argentina Fil: Velasco Zamora, Jorge. Fundación Articular; Argentina Fil: Perone, Marcelo Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigación en Biomedicina de Buenos Aires; Argentina Fil: Dewey, Ricardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Instituto de Investigaciones Biotecnológicas - Instituto Tecnológico Chascomús. Instituto de Investigaciones Biotecnológicas (sede Chascomús); Argentina. Universidad Nacional de San Martín; Argentina |
| Databáze: | OpenAIRE |
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