Next-Generation Sequencing of Coccidioides immitis Isolated during Cluster Investigation
| Autor: | Tracy Moses, James S. Beckstrom-Sternberg, Tom Chiller, John D. Carpten, Shripad Sinari, Paul Keim, James A. Schupp, S. Arunmozhi Balajee, Ashley Peterson, David M. Engelthaler, Joshua Colvin, Dawn Terashita, Elizabeth M. Driebe, Alexis Christoforides, Waibhav Tembe, Stephen M. Beckstrom-Sternberg, John V. Pearson |
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| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Microbiology (medical)
Genotype Epidemiology Coccidioides immitis lcsh:Medicine Polymorphism Single Nucleotide molecular epidemiology DNA sequencing Mycological Typing Techniques lcsh:Infectious and parasitic diseases 03 medical and health sciences Species Specificity Cluster Analysis Humans Coccidioides lcsh:RC109-216 Typing DNA Fungal Genotyping Phylogeny 030304 developmental biology Genetics next generation sequencing 0303 health sciences Coccidioidomycosis biology Molecular epidemiology 030306 microbiology Research lcsh:R Fungi Organ Transplantation Sequence Analysis DNA biology.organism_classification 3. Good health Infectious Diseases genotyping whole genome sequence typing Genome Fungal Sequence Alignment |
| Zdroj: | Emerging Infectious Diseases, Vol 17, Iss 2, Pp 227-232 (2011) Emerging Infectious Diseases |
| ISSN: | 1080-6059 1080-6040 |
| Popis: | Next-generation sequencing enables use of whole-genome sequence typing (WGST) as a viable and discriminatory tool for genotyping and molecular epidemiologic analysis. We used WGST to confi rm the linkage of a cluster of Coccidioides immitis isolates from 3 patients who received organ transplants from a single donor who later had positive test results for coccidioidomycosis. Isolates from the 3 patients were nearly genetically identical (a total of 3 singlenucleotide polymorphisms identifi ed among them), thereby demonstrating direct descent of the 3 isolates from an original isolate. We used WGST to demonstrate the genotypic relatedness of C. immitis isolates that were also epidemiologically linked. Thus, WGST offers unique benefi ts to public health for investigation of clusters considered to be linked to a single source. |
| Databáze: | OpenAIRE |
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