Design of flow chamber with electronic cell volume capability and light detection optics for multilaser flow cytometry
| Autor: | M. A. MacCollum, W. H. Schuette, Stanley E. Shackney, H. W. Tipton, F. A. Plowman, C. A. Smith |
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| Rok vydání: | 1984 |
| Předmět: |
Erythrocytes
Materials science Lymphoma Cells Biophysics Signal Pathology and Forensic Medicine law.invention Mice Endocrinology Optics law Animals Fluidics Erythrocyte Volume business.industry Lasers Cell Biology Hematology Flow Cytometry Laser Lens (optics) Cuvette Flow (mathematics) Electromagnetic shielding business Reduction (mathematics) Chickens Cell Division |
| Zdroj: | Cytometry. 5:652-656 |
| ISSN: | 1097-0320 0196-4763 |
| DOI: | 10.1002/cyto.990050616 |
| Popis: | A multibeam optical detection system has been developed with a high optical efficiency, achieved through a reduction in the number of optical interfaces employed in the system. This reduction is made possible by a combination of employing simple lenses, gluing the objective lens directly upon the face of the flow cuvette and the extraction of only one fluorescence signal from each laser beam. A modified flow chamber is also described that includes fluidic resistance elements for the elimination of most of the electric shielding normally associated with electronic cell volume measurements. |
| Databáze: | OpenAIRE |
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