Novel Spectroscopic and Electrochemical Sensors and Nanoprobes for the Characterization of Food and Biological Antioxidants

Autor: Mustafa Bener, Ayşe Nur Önem, Erol Erçağ, Ayşem Üzer, Burcu Bekdeşer, Reşat Apak, Sema Demirci Çekiç, Şener Sağlam, Ziya Can, Saliha Esin Çelik
Rok vydání: 2017
Předmět:
Antioxidant
Silver
DMPD oxidant assay
medicine.medical_treatment
Nanoparticle
Metal Nanoparticles
Nanotechnology
02 engineering and technology
Review
antioxidant capacity
engineering.material
lcsh:Chemical technology
Electrochemistry
01 natural sciences
Biochemistry
Silver nanoparticle
Antioxidants
Analytical Chemistry
Nanosensor
medicine
lcsh:TP1-1185
Electrical and Electronic Engineering
Surface plasmon resonance
Instrumentation
CUPRAC antioxidant assay
Chemistry
010401 analytical chemistry
nanoprobes
oxidative status
021001 nanoscience & nanotechnology
Atomic and Molecular Physics
and Optics
0104 chemical sciences
Nanostructures
Colloidal gold
Spectrophotometry
engineering
Noble metal
Gold
Microscopy
Electrochemical
Scanning
colorimetric and electrochemical sensors
0210 nano-technology
Oxidation-Reduction
Food Analysis
Zdroj: Sensors (Basel, Switzerland)
Sensors, Vol 18, Iss 1, p 186 (2018)
ISSN: 1424-8220
Popis: Since an unbalanced excess of reactive oxygen/nitrogen species (ROS/RNS) causes various diseases, determination of antioxidants that can counter oxidative stress is important in food and biological analyses. Optical/electrochemical nanosensors have attracted attention in antioxidant activity (AOA) assessment because of their increased sensitivity and selectivity. Optical sensors offer advantages such as low cost, flexibility, remote control, speed, miniaturization and on-site/in situ analysis. Electrochemical sensors using noble metal nanoparticles on modified electrodes better catalyze bioelectrochemical reactions. We summarize the design principles of colorimetric sensors and nanoprobes for food antioxidants (including electron-transfer based and ROS/RNS scavenging assays) and important milestones contributed by our laboratory. We present novel sensors and nanoprobes together with their mechanisms and analytical performances. Our colorimetric sensors for AOA measurement made use of cupric-neocuproine and ferric-phenanthroline complexes immobilized on a Nafion membrane. We recently designed an optical oxidant/antioxidant sensor using N,N-dimethyl-p-phenylene diamine (DMPD) as probe, from which ROS produced colored DMPD-quinone cationic radicals electrostatically retained on a Nafion membrane. The attenuation of initial color by antioxidants enabled indirect AOA estimation. The surface plasmon resonance absorption of silver nanoparticles as a result of enlargement of citrate-reduced seed particles by antioxidant addition enabled a linear response of AOA. We determined biothiols with Ellman reagent−derivatized gold nanoparticles.
Databáze: OpenAIRE
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