| Popis: |
Antibody against PR toxin was produced after immunizing rabbits with an immunogen prepared by conjugation of PR toxin to bovine serum albumin by a reductive alkylation method. A competitive radioimmunoassay (RIA) was used to determine the antibody specificity. The concentration causing 50% inhibition of binding of 3H-tetrahydro-PR toxin to the antibody by unlabeled PR toxin, tetrahydro-PR toxin, PR imine, eremofortin C (EC), acetyl-EC (Ac-EC), eremofortin D (ED) and eremofortin A (EA) were 7, 10, 5, 15, 50, 500 and 800 ng/assay, respectively; for PR alcohol and eremofortin B (EB), the concentration was greater than 10,000 ng/assay. The practical application of using this antibody for RIA of PR toxin was tested by spiking cheese with the toxin. PR toxin was then extracted with ethyl acetate, and analyzed by RIA. The overall recovery for 20 samples with 0.1 to 50 ppm of PR toxin was 93%. |
