The chicken pituitary-specific transcription factor Pit-1 is involved in the hypothalamic regulation of pituitary hormones

Autor: K. Pals, C. Denef, Okanlawon Onagbesan, Eddy Decuypere, B. De Groef, P. Van As, Veerle Bruggeman, Kristel Janssens, Veerle Darras
Jazyk: angličtina
Rok vydání: 2006
Předmět:
Male
medicine.medical_specialty
Pituitary gland
Hypothalamo-Hypophyseal System
endocrine system
endocrine system diseases
receptor
thyroid-stimulating hormone
Thyrotropin-releasing hormone
Thyrotropin
beta Subunit
Biology
Animal Breeding and Genomics
Cell Line
Thyroid-stimulating hormone
Internal medicine
sequence-analysis
Gene expression
medicine
Animals
Fokkerij en Genomica
RNA
Messenger
Thyrotropin-Releasing Hormone
DNA Primers
General Veterinary
Reverse Transcriptase Polymerase Chain Reaction
molecular-cloning
Thyroid
gene-expression
Prolactin
secretion
Endocrinology
medicine.anatomical_structure
Gene Expression Regulation
Hypothalamus
Growth Hormone
Pituitary Gland
growth-hormone
WIAS
beta-subunit
Transcription Factor Pit-1
protein
Chickens
hormones
hormone substitutes
and hormone antagonists
Hormone
thyrotropin-releasing-hormone
Zdroj: Acta Veterinaria Hungarica, 54(4), 455-471
Acta Veterinaria Hungarica 54 (2006) 4
ISSN: 0236-6290
Popis: Pit-1 is a pituitary-specific POU-domain DNA binding factor, which binds to and trans-activates promoters of growth hormone- (GH), prolactin- (PRL) and thyroid stimulating hormone-beta- (TSHbeta) encoding genes. Thyrotropin-releasing hormone (TRH) is located in the hypothalamus and stimulates TSH, GH and PRL release from the pituitary gland. In the present study, we successfully used the cell aggregate culture system for chicken pituitary cells to study the effect of TRH administration on the ggPit-l* (chicken Pit-1), GH and TSHbeta mRNA expression in vitro. In pituitary cell aggregates of 11-day-old male broiler chicks the ggPit-l * mRNA expression was significantly increased following TRH administration, indicating that the stimulatory effects of TRH on several pituitary hormones are mediated via its effect on the ggPit-l* gene expression. Therefore, a semiquantitative RT-PCR method was used to detect possible changes in GH and TSHbeta mRNA levels. TRH affected both the GH and TSHbeta mRNA levels. The results of this in vitro study reveal that ggPit-1 * has a role in mediating the stimulatory effects of TRH on pituitary hormones like GH and TSHbeta in the chicken pituitary.
Databáze: OpenAIRE
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