Genome-Wide Association Study for Maize Leaf Cuticular Conductance Identifies Candidate Genes Involved in the Regulation of Cuticle Development
| Autor: | Isabel Molina, Michael G. Miller, Laurie G. Smith, Michael A. Gore, Miguel F. Vasquez, Michael J. Scanlon, Ethan L. Stewart, James Chamness, Matheus Baseggio, Nicholas Kaczmar, Susanne Matschi, Pengfei Qiao, Meng Lin |
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| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
Genome-wide association study Candidate gene Cuticle Drought tolerance Cutin QH426-470 Investigations Biology Zea mays 01 natural sciences 03 medical and health sciences Gene Expression Regulation Plant Genetics Molecular Biology Gene Cell wall modification Genetics (clinical) Cuticular conductance 030304 developmental biology Laser capture microdissection 0303 health sciences Human Genome fungi food and beverages RNA RNA sequencing Plant Droughts Cell biology Plant Leaves Gene Expression Regulation Waxes Whole-genome prediction Biotechnology 010606 plant biology & botany |
| Zdroj: | G3 (Bethesda, Md.), vol 10, iss 5 G3: Genes|Genomes|Genetics G3: Genes, Genomes, Genetics, Vol 10, Iss 5, Pp 1671-1683 (2020) |
| ISSN: | 2160-1836 |
| DOI: | 10.1534/g3.119.400884 |
| Popis: | The cuticle, a hydrophobic layer of cutin and waxes synthesized by plant epidermal cells, is the major barrier to water loss when stomata are closed at night and under water-limited conditions. Elucidating the genetic architecture of natural variation for leaf cuticular conductance (gc) is important for identifying genes relevant to improving crop productivity in drought-prone environments. To this end, we conducted a genome-wide association study ofgcof adult leaves in a maize inbred association panel that was evaluated in four environments (Maricopa, AZ, and San Diego, CA in 2016 and 2017). Five genomic regions significantly associated withgcwere resolved to seven plausible candidate genes (ISTL1, two SEC14 homologs, cyclase-associated protein, a CER7 homolog, GDSL lipase, and β-D-XYLOSIDASE 4). These candidates are potentially involved in cuticle biosynthesis, trafficking and deposition of cuticle lipids, cutin polymerization, and cell wall modification. Laser microdissection RNA sequencing revealed that all these candidate genes, with the exception of the CER7 homolog, were expressed in the zone of the expanding adult maize leaf where cuticle maturation occurs. With direct application to genetic improvement, moderately high average predictive abilities were observed for whole-genome prediction ofgcin locations (0.46 and 0.45) and across all environments (0.52). The findings of this study provide novel insights into the genetic control ofgcand have the potential to help breeders more effectively develop drought-tolerant maize for target environments.Article summaryThe cuticle serves as the major barrier to water loss when stomata are closed at night and under water-limited conditions and potentially relevant to drought tolerance in crops. We performed a genome-wide association study to elucidate the genetic architecture of natural variation for maize leaf cuticular conductance. We identified epidermally expressed candidate genes that are potentially involved in cuticle biosynthesis, trafficking and deposition, cutin polymerization, and cell wall modification. Finally, we observed moderately high predictive abilities for whole-genome prediction of leaf cuticular conductance. Collectively, these findings may help breeders more effectively develop drought-tolerant maize. |
| Databáze: | OpenAIRE |
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