Synthesis and Enzymatic Stability of Phosphodiester-Linked Peptide−Oligonucleotide Hybrids
| Autor: | Maite Beltrán, Marta Maseda, Enrique Pedroso, Anna Grandas, Jordi Robles, Maria Concernau |
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| Rok vydání: | 1997 |
| Předmět: |
animal structures
Stereochemistry Oligonucleotides Biomedical Engineering Pharmaceutical Science Bioengineering Peptide Hydrolysis Drug Stability Chromatography High Pressure Liquid Pharmacology chemistry.chemical_classification integumentary system Phosphoric Diester Hydrolases Lability Oligonucleotide Chemistry Organic Chemistry Esters Alkaline Phosphatase Amino acid Enzyme Phosphodiesterase I Phosphodiester bond Oligopeptides After treatment Biotechnology |
| Zdroj: | Bioconjugate Chemistry. 8:785-788 |
| ISSN: | 1520-4812 1043-1802 |
| DOI: | 10.1021/bc970051u |
| Popis: | Nucleopeptides Ac-Tyr(p3' dACGT)-Ala-Phe-Gly-NH2, Ac-Thr(p3'dACGT)-Ala-Phe-Gly-OH, Ac-Ser(p3'dACGT)-Ala-Phe-Gly-OH, and Phac-Hse(p3'dACGT)-Ala-Phe-Gly-OH, in which the 3'-end of a tetradeoxyribonucleotide is linked by a phosphodiester bond to a hydroxylated amino acid, were synthesized using a stepwise solid-phase methodology to study the influence of the linking amino acid on their stability to 3'-exonucleases. HPLC analysis of the reaction crudes after treatment of each nucleopeptide with snake venom phosphodiesterase showed that the lability of the amino acid-nucleoside linkage increases in the order Thr < Ser < Hse < Tyr. |
| Databáze: | OpenAIRE |
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