One-pot conjugated linoleic acid production from castor oil by Rhizopus oryzae lipase and resting cells of Lactobacillus plantarum
| Autor: | Farah Naz Talpur, Hassan Imran Afridi, Aysun Cebeci Aydin, Muhammad Ali Surhio, Abid Ali Khaskheli, Sana Jawaid |
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| Přispěvatelé: | AGÜ, Mühendislik Fakültesi, Malzeme Bilimi ve Nanoteknoloji Mühendisliği Bölümü |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine Castor Oil Conjugated linoleic acid Cost-Benefit Analysis Rhizopus oryzae 01 natural sciences Applied Microbiology and Biotechnology Biochemistry conjugated linoleic acid Analytical Chemistry Catalysis 03 medical and health sciences chemistry.chemical_compound 010608 biotechnology medicine castor oil Spectral analysis Linoleic Acids Conjugated Food science Lipase Molecular Biology biology Hydrolysis Probiotics Organic Chemistry Substrate (chemistry) food and beverages General Medicine Hydrogen-Ion Concentration biology.organism_classification Kinetics 030104 developmental biology chemistry Castor oil biology.protein Lactobacillus plantarum Rhizopus medicine.drug Biotechnology |
| Popis: | Abid Ali Khaskheli greatly acknowledges the National Centre of Excellence in Analytical Chemistry, University of Sindh for providing monthly stipend to carry out this research work. No further funding was available. Conjugated linoleic acid (CLA) has attracted as novel type of fatty acids having unusual health-promoting properties such as anticarcinogenic and antiobesitic effects. The present work employed castor oil as substrate for one-pot production of CLA using washed cells of Lactobacillus plantarum (L. plantarum) and lipases as catalysts. Among the screened lipases, the lipase Rhizopus oryzae (ROL) greatly assisted resting cells to produce CLA. Mass spectral analysis of the product showed that two major isomers of CLA were produced in the reaction mixture i.e. cis-9, trans-11 56.55% and trans-10, cis-12 43.45%. Optimum factors for CLA synthesis were found as substrate concentration (8mg/mL), pH (6.5), washed cell concentration (12% w/v), and incubation time of 20h. Hence, the combination of ROL with L. plantarum offers one pot production of CLA selectively using castor oil as a cost-effective substrate. National Centre of Excellence in Analytical Chemistry, University of Sindh |
| Databáze: | OpenAIRE |
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