Spectrophotometric determination of alogliptin in bulk and tablet dosage form using bromate–bromide mixture as brominating agent
| Autor: | T.V. Reddy, A.V.V.N.K. Sunil Kumar, C.B. Sekharan |
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| Rok vydání: | 2017 |
| Předmět: |
Detection limit
Accuracy and precision Multidisciplinary Bromine Chromatography Physics and Astronomy (miscellaneous) Chemistry 010401 analytical chemistry chemistry.chemical_element Bromate 030226 pharmacology & pharmacy 01 natural sciences Biochemistry Genetics and Molecular Biology (miscellaneous) Dosage form 0104 chemical sciences Absorbance 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Chemistry (miscellaneous) Bromide Computer Science (miscellaneous) Alogliptin |
| Zdroj: | Karbala International Journal of Modern Science. |
| ISSN: | 2405-609X |
| DOI: | 10.1016/j.kijoms.2016.12.002 |
| Popis: | Two simple and sensitive visible spectrophotometric methods (A and B) have been developed and validated for the determination of alogliptin in bulk and tablet dosage forms. The methods are based on the bromination of alogliptin using bromine produced by the action of HCl on the bromate–bromide mixture. The residual bromine is determined with a fixed amount of either methyl orange and measuring the absorbance at 505 nm (method A) or methylene blue and measuring the absorbance at 720 nm (method B). Linearity, accuracy and precision were found to be acceptable over the concentration ranges of 1–10 μg mL −1 and 2.5–12.5 μg mL −1 for method A and method B, respectively. The detection limits were 0.115 μg mL −1 and 0.210 μg mL −1 for method A and method B, respectively. The accuracy of the proposed methods was assessed by recovery studies and the percent recoveries of aloglitpin were found to be 99.91 ± 0.126%–99.99 ± 0.168% for method A and 99.86 ± 0.170%–99.98 ± 0.193% for method B. The methods were successfully applied to the determination of alogliptin in tablets with percentage recovery of 99.84 ± 0.139%–100.20 ± 0.625% (method A) and 99.96 ± 0.351%–10.320 ± 0.422% (method B). The optimized methods were fully validated and proved to be specific, robust, precise and accurate for the quality control of the alogliptin in their pharmaceutical formulations. |
| Databáze: | OpenAIRE |
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