Feasibility of a 3D human airway epithelial model to study respiratory absorption
| Autor: | C. Frieke Kuper, Astrid A. Reus, Harm T. Jansen, Jos van Triel, Yvonne C.M. Staal, Wilfred J. M. Maas, Samuel Constant |
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| Rok vydání: | 2013 |
| Předmět: |
Respiratory Mucosa
Adult Male Models Anatomic Respiratory Transport Respiratory Tract Diseases Bronchi Absorption (skin) Toxicology Absorption medicine Humans Respiratory system Aged Reproducibility Chromatography Chemistry Epithelial Cells General Medicine Middle Aged Atenolol Epithelium Asthma medicine.anatomical_structure Biochemistry Pharmaceutical Preparations Feasibility Studies Female Mannitol Algorithms medicine.drug |
| Zdroj: | Toxicology in vitro : an international journal published in association with BIBRA. 28(2) |
| ISSN: | 1879-3177 |
| Popis: | The respiratory route is an important portal for human exposure to a large variety of substances. Consequently, there is an urgent need for realistic in vitro strategies for evaluation of the absorption of airborne substances with regard to safety and efficacy assessment. The present study investigated feasibility of a 3D human airway epithelial model to study respiratory absorption, in particular to differentiate between low and high absorption of substances. Bronchial epithelial models (MucilAir™), cultured at the air–liquid interface, were exposed to eight radiolabeled model substances via the apical epithelial surface. Absorption was evaluated by measuring radioactivity in the apical compartment, the epithelial cells and the basolateral culture medium. Antipyrine, caffeine, naproxen and propranolol were highly transported across the epithelial cell layer (>5%), whereas atenolol, mannitol, PEG-400 and insulin were limitedly transported ( |
| Databáze: | OpenAIRE |
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