NMR probing and visualization of correlated structural fluctuations in intrinsically disordered proteins
| Autor: | Thomas C. Schwarz, Dennis Kurzbach, Robert Konrat, Agathe Vanas, Andrea G. Flamm, Andreas Beier, Gerald Platzer |
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| Rok vydání: | 2017 |
| Předmět: |
Models
Molecular 0301 basic medicine 030102 biochemistry & molecular biology Chemistry Brain Acid Soluble Protein 1 Relaxation (NMR) Membrane Proteins General Physics and Astronomy Nerve Tissue Proteins Nanotechnology Intrinsically disordered proteins Protein Structure Tertiary Intrinsically Disordered Proteins Repressor Proteins 03 medical and health sciences Paramagnetism 030104 developmental biology Nuclear magnetic resonance Correlation analysis Humans Osteopontin Statistical analysis Physical and Theoretical Chemistry Nuclear Magnetic Resonance Biomolecular |
| Zdroj: | Physical Chemistry Chemical Physics. 19:10651-10656 |
| ISSN: | 1463-9084 1463-9076 |
| DOI: | 10.1039/c7cp00430c |
| Popis: | A novel statistical analysis of paramagnetic relaxation enhancement (PRE) and paramagnetic relaxation interference (PRI) based nuclear magnetic resonance (NMR) data is proposed based on the computation of correlation matrices. The technique is demonstrated with an example of the intrinsically disordered proteins (IDPs) osteopontin (OPN) and brain acid soluble protein 1 (BASP1). The correlation analysis visualizes in detail the subtleties of conformational averaging in IDPs and highlights the presence of correlated structural fluctuations of individual sub-domains in IDPs. |
| Databáze: | OpenAIRE |
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