Fetal wound healing: An in vitro explant model
| Autor: | N. Scott Adzick, H. Paul Ehrlich, Michael T. Longaker, John W. Siebert, D. Andrew R. Burd, Carolyn C. Compton, Michael R. Harrison |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
medicine.medical_specialty
Pathology Cicatrix Fetus In vivo medicine Animals Cells Cultured Skin Wound Healing Sheep integumentary system Epidermis (botany) Sheep skin business.industry General Medicine In vitro Culture Media Surgery Pediatrics Perinatology and Child Health Fetal wound healing Wound healing business Explant culture |
| Zdroj: | Journal of Pediatric Surgery. 25:898-901 |
| ISSN: | 0022-3468 |
| DOI: | 10.1016/0022-3468(90)90199-j |
| Popis: | The ability of fetal skin wounds to heal without scar formation is remarkable. The mechanisms that endow the fetus with this unique healing ability remain unknown. We have developed an in vitro explant model using fetal sheep skin to investigate fetal wound healing. This model eliminates the complex systemic mechanisms that modulate in vivo wound healing. We demonstrated that using an enriched medium, midgestation fetal sheep skin explants following wounding reepithelialized within 4 days. By 7 days after wounding the confluent epidermis was thicker, but the dermal wound remained open. This model demonstrates that it is possible to achieve conditions in culture that maintain tissue viability and support reepithelialization. This model may allow us to resolve some of the individual components that participate in the process of scarless fetal skin healing. |
| Databáze: | OpenAIRE |
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