Molecular and functional characterization of a soluble form of oncostatin M/interleukin-31 shared receptor
| Autor: | Emilie Venereau, Caroline Diveu, Sylvie Chevalier, Hugues Gascan, Elisa Ravon, Josy Froger, François Rousseau, Linda Grimaud |
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| Rok vydání: | 2006 |
| Předmět: |
Glycoside Hydrolases
Receptors OSM-LIF Amino Acid Motifs Molecular Sequence Data Leukemia inhibitory factor receptor Oncostatin M Biology Biochemistry Cell Line Tumor Cytokine Receptor gp130 Humans Tissue Distribution Amino Acid Sequence Cytokine binding Receptor Molecular Biology Base Sequence Interleukins fungi Oncostatin M receptor Cell Biology Glycoprotein 130 Molecular biology Protein Structure Tertiary Alternative Splicing Interleukin 31 Interleukin-21 receptor biology.protein Protein Binding |
| Zdroj: | The Journal of biological chemistry. 281(48) |
| ISSN: | 0021-9258 |
| Popis: | Activation of the signaling transduction pathways mediated by oncostatin M (OSM) requires the binding of the cytokine to either type I OSM receptor (leukemia inhibitory factor receptor/gp130) or to type II OSM receptor (OSMR/gp130). In the present work we have developed an enzyme-linked immunosorbent assay detecting a soluble form of OSMR (sOSMR) secreted by glioblastoma, hepatoma, and melanoma tumor cell lines. sOSMR was also present in sera of healthy individuals, with increased levels in multiple myeloma. Molecular cloning of a corresponding cDNA was carried out, and it encoded for a 70-kDa protein consisting of a half cytokine binding domain containing the canonical WSXWS motif, an immunoglobulin-like domain, and the first half of a second cytokine binding domain with cysteines in fixed positions. Analysis of the soluble receptor distribution revealed a preferential expression in lung, liver, pancreas, and placenta. sOSMR was able to bind OSM and interleukin-31 when associated to soluble gp130 or soluble interleukin-31R, respectively, and to neutralize both cytokine properties. We have also shown that OSM could positively regulate the synthesis of its own soluble receptor in tumor cells. |
| Databáze: | OpenAIRE |
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