An RNA-interference screen in Drosophila to identify ZAD-containing C2H2 zinc finger genes that function in female germ cells
| Autor: | Helen K. Salz, Laura Shapiro-Kulnane, Oscar B Bautista |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
AcademicSubjects/SCI01140
AcademicSubjects/SCI00010 M1PB Biology AcademicSubjects/SCI01180 gametogenesis CG17802 reproduction 03 medical and health sciences CG320020 0302 clinical medicine RNA interference Genetics medicine Animals CYS2-HIS2 Zinc Fingers Drosophila Proteins Gene family Molecular Biology Gene multigene family Genetics (clinical) 030304 developmental biology 0303 health sciences Gene knockdown C2H2 Zinc Finger oogenesis Mutant Screen Report trem Zinc Fingers odj Zif Phenotype Cell biology Germ Cells medicine.anatomical_structure RNA AcademicSubjects/SCI00960 CG4936 Drosophila Female hang 030217 neurology & neurosurgery Germ cell Function (biology) |
| Zdroj: | G3: Genes|Genomes|Genetics |
| ISSN: | 2160-1836 |
| Popis: | The zinc finger-associated domain (ZAD) is present in over 90 C2H2 zinc finger (ZNF) proteins. Despite their abundance, only a few ZAD-ZNF genes have been characterized to date. Here, we systematically analyze the function of 68 ZAD-ZNF genes in Drosophila female germ cells by performing an in vivo RNA-interference screen. We identified eight ZAD-ZNF genes required for oogenesis, and based on further characterization of the knockdown phenotypes, we uncovered defects broadly consistent with functions in germ cell specification and/or survival, early differentiation, and egg chamber maturation. These results provide a candidate pool for future studies aimed at functionalization of this large but poorly characterized gene family. |
| Databáze: | OpenAIRE |
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