Carrageenan polysaccharides and oligosaccharides with distinct immunomodulatory activities in murine microglia BV-2 cells
| Autor: | Wang Guangqiang, Xiong Zhiqiang, Syuan-Yu Lin, Kee-Ching G. Jeng, Lianzhong Ai, Yung-Chin Chung, Phoency F.-H. Lai |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Lipopolysaccharides
0301 basic medicine Lipopolysaccharide medicine.medical_treatment Dose-Response Relationship Immunologic Oligosaccharides Chemical Fractionation Carrageenan Polysaccharide Biochemistry Mice 03 medical and health sciences chemistry.chemical_compound 0404 agricultural biotechnology Polysaccharides Structural Biology medicine Animals Molecular Biology Cell Line Transformed chemistry.chemical_classification Interleukin-6 Tumor Necrosis Factor-alpha Hydrolysis Anti-Inflammatory Agents Non-Steroidal 04 agricultural and veterinary sciences General Medicine Oligosaccharide 040401 food science Molecular biology In vitro 030104 developmental biology Cytokine chemistry Cell culture Tumor necrosis factor alpha Microglia |
| Zdroj: | International Journal of Biological Macromolecules. 120:633-640 |
| ISSN: | 0141-8130 |
| DOI: | 10.1016/j.ijbiomac.2018.08.151 |
| Popis: | This study was to investigate the anti-inflammatory activities in vitro of various carrageenans ( Car ) fractions (κ-, ι-, and λ-types) with well characterized molecular properties, using murine microglia BV-2 cell line treated with lipopolysaccharide (LPS) as model. It is indicated that pretreatments with the oligosaccharide fractions from κ- or ι-carrageenan acid hydrolysates ( κ- and ι-CarAOS , respectively) at 125–500 μg/mL significantly and dose-dependently decreased the levels of tumor necrosis factor α (TNF-α) secreted from LPS-treated BV-2 cells, showing promisingly anti-inflammatory effects. Differently, pretreatments of most of polymeric carrageenans at 250–500 μg/mL significantly increased the TNF-α level, implying the co-inflammatory effects with LPS. The co-inflammatory effectiveness of pure carrageenans at 125 μg/mL was notable for λ-Car , followed by ι-Car , and insignificantly for κ-Car . Generally, cytokine TNF-α was a more sensitive biomarker to the presence of carrageenans than was the IL-6. The TNF-α level varied greatly at a low carrageenan concentration (125 μg/mL) and high polymer percentage ( e.g. purified κ- and ι-Car ). Conclusively, the anti-inflammatory effects on LPS-treated BV-2 cells could be attenuated by pretreatments with κ- and ι-CarAOS at 125–500 μg/mL. |
| Databáze: | OpenAIRE |
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