Lipid Self-Assemblies under the Atomic Force Microscope
| Autor: | Alicia Alonso, Félix M. Goñi, Aritz B. García-Arribas |
|---|---|
| Přispěvatelé: | Universidad del País Vasco, Eusko Jaurlaritza, European Commission, Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), Fundación Biofísica Bizkaia, García Arribas, Aritz B., Alonso, Alicia |
| Rok vydání: | 2021 |
| Předmět: |
QH301-705.5
Lipid Bilayers Biophysics Review Microscopy Atomic Force Catalysis Inorganic Chemistry Membrane Lipids Atomic force microscopy Membrane Microdomains Phase (matter) Lipid assemblies Supported planar bilayers Biology (General) Physical and Theoretical Chemistry Lipid bilayer Cytoskeleton QD1-999 Molecular Biology Phospholipids Spectroscopy Sphingolipids Chemistry Bilayer Cell Membrane Organic Chemistry General Medicine Lipids Sphingolipid Computer Science Applications Cell membranes Membrane Biophysical Process Model membranes lipids (amino acids peptides and proteins) Nanodomains Membrane biophysics |
| Zdroj: | International Journal of Molecular Sciences International Journal of Molecular Sciences, Vol 22, Iss 10085, p 10085 (2021) |
| ISSN: | 1422-0067 |
| DOI: | 10.3390/ijms221810085 |
| Popis: | Lipid model membranes are important tools in the study of biophysical processes such as lipid self-assembly and lipid-lipid interactions in cell membranes. The use of model systems to adequate and modulate complexity helps in the understanding of many events that occur in cellular membranes, that exhibit a wide variety of components, including lipids of different subfamilies (e.g., phospholipids, sphingolipids, sterols…), in addition to proteins and sugars. The capacity of lipids to segregate by themselves into different phases at the nanoscale (nanodomains) is an intriguing feature that is yet to be fully characterized in vivo due to the proposed transient nature of these domains in living systems. Model lipid membranes, instead, have the advantage of (usually) greater phase stability, together with the possibility of fully controlling the system lipid composition. Atomic force microscopy (AFM) is a powerful tool to detect the presence of meso- and nanodomains in a lipid membrane. It also allows the direct quantification of nanomechanical resistance in each phase present. In this review, we explore the main kinds of lipid assemblies used as model membranes and describe AFM experiments on model membranes. In addition, we discuss how these assemblies have extended our knowledge of membrane biophysics over the last two decades, particularly in issues related to the variability of different model membranes and the impact of supports/cytoskeleton on lipid behavior, such as segregated domain size or bilayer leaflet uncoupling. A.B.G.A. was a post-doctoral scientist supported by the University of the Basque Country and the Basque Government. This work was supported in part by grants from the Spanish Ministry of Economy (grant FEDER MINECO PGC2018-099857-B-I00) and the Basque Government (grants no. IT1264-19 and IT1270-19), as well as Fundación Biofísica Bizkaia and the Basque Excellence Research Centre (BERC) program of the Basque Government. |
| Databáze: | OpenAIRE |
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