Multiplex PCR using 16S rRNA gene-targeted primers for the identification of bifidobacteria from human origin
| Autor: | Elisabeth Singer, Catherine Mullié, Daniel Izard, Marie-Françoise Odou, Marie-Benedicte Romond |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
DNA
Bacterial Microbiology Polymerase Chain Reaction law.invention Feces fluids and secretions law RNA Ribosomal 16S Multiplex polymerase chain reaction Genetics Bifidobacteriales Infections Humans Multiplex Molecular Biology Ribosomal DNA Polymerase Polymerase chain reaction Phylogeny Bifidobacterium DNA Primers biology food and beverages Actinomycetaceae Infant biology.organism_classification 16S ribosomal RNA Molecular biology biology.protein |
| Zdroj: | FEMS microbiology letters. 222(1) |
| ISSN: | 0378-1097 |
| Popis: | Three multiplex polymerase chain reactions (PCRs) targeted on Bifidobacterium and related species were designed to identify human species. The selected primers yielded amplified products of various sizes, each specific for a species. Three to four pairs were gathered in one PCR reaction and their specificity under multiplex conditions was confirmed using DNA from 26 reference strains. Using this technique on unidentified faecal strains, B. bifidum, B. longum and B. breve species were commonly recovered in infants while B. adolescentis, B. catenulatum/B. pseudocatenulatum continuum and B. longum species were predominant in adults. Thus, a single PCR can provide the assignment of a strain to one these species, reducing the number of PCR reactions and hands-on time for the identification of human isolates of bifidobacteria. Moreover, this technique is also applicable for the in situ detection of bifidobacteria in DNA extracts from human stools. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text