Effect of 3-bromopyruvate acid on the redox equilibrium in non-invasive MCF-7 and invasive MDA-MB-231 breast cancer cells
| Autor: | Agnieszka Gajewska, Mirosław Soszyński, Izabela Sadowska-Bartosz, Martyna Wojtala, Ewa Kwiatkowska, Grzegorz Bartosz |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Antioxidant Physiology medicine.medical_treatment Glutathione reductase Breast Neoplasms medicine.disease_cause 03 medical and health sciences chemistry.chemical_compound medicine Humans Neoplasm Invasiveness Pyruvates skin and connective tissue diseases Glutathione Transferase chemistry.chemical_classification Reactive oxygen species Dose-Response Relationship Drug Glutathione peroxidase Cell Biology Glutathione Molecular biology Warburg effect Neoplasm Proteins Glutathione Reductase 030104 developmental biology chemistry Cancer cell Immunology MCF-7 Cells Female Reactive Oxygen Species Oxidation-Reduction Oxidative stress |
| Zdroj: | Journal of Bioenergetics and Biomembranes. 48:23-32 |
| ISSN: | 1573-6881 0145-479X |
| Popis: | Novel approaches to cancer chemotherapy employ metabolic differences between normal and tumor cells, including the high dependence of cancer cells on glycolysis ("Warburg effect"). 3-Bromopyruvate (3-BP), inhibitor of glycolysis, belongs to anticancer drugs basing on this principle. 3-BP was tested for its capacity to kill human non-invasive MCF-7 and invasive MDA-MB-231 breast cancer cells. We found that 3-BP was more toxic for MDA-MB-231 cells than for MCF-7 cells. In both cell lines, a statistically significant decrease of ATP and glutathione was observed in a time- and 3-BP concentration-dependent manner. Transient increases in the level of reactive oxygen species and reactive oxygen species was observed, more pronounced in MCF-7 cells, followed by a decreasing tendency. Activities of glutathione peroxidase, glutathione reductase (GR) and glutathione S-transferase (GST) decreased in 3-BP treated MDA-MB-231 cells. For MCF-7 cells decreases of GR and GST activities were noted only at the highest concentration of 3-BP.These results point to induction of oxidative stress by 3-BP via depletion of antioxidants and inactivation of antioxidant enzymes, more pronounced in MDA-MB-231 cells, more sensitive to 3-BP. |
| Databáze: | OpenAIRE |
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