Mass spectrometry-based fragmentation as an identification tool in lignomics
| Autor: | Hoon Kim, Claudiu Niculaes, Eric Messens, Dirk Inzé, Kris Morreel, Geert Goeminne, Fachuang Lu, Oana Dima, John Ralph, Ruben Vanholme, Wout Boerjan, Takuya Akiyama |
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| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
DOWN-REGULATION
ELECTROSPRAY-IONIZATION Electrospray ionization LIGNIN BIOSYNTHESIS METABOLISM Mass spectrometry METABOLOMICS Lignin Mass Spectrometry Analytical Chemistry chemistry.chemical_compound Metabolomics Fragmentation (mass spectrometry) REVEALS Metabolome POPLAR XYLEM Chromatography High Pressure Liquid O-METHYLTRANSFERASE Phenylpropanoid NEGATIVE-IONS Biology and Life Sciences OLIGOLIGNOLS chemistry Biochemistry Gases Monolignol |
| Zdroj: | ANALYTICAL CHEMISTRY |
| ISSN: | 0003-2700 |
| Popis: | The ensemble of all phenolics for which the biosynthesis is coregulated with lignin biosynthesis, i.e., metabolites from the general phenylpropanoid, monolignol, and (neo)-lignan biosynthetic pathways and their derivatives, as well as the lignin oligomers, is coined the lignome. In lignifying tissues, the lignome comprises a significant portion of the metabolome. However, as is true for metabolomics in general, the structural elucidation of unknowns represents the biggest challenge in characterizing the lignome. To minimize the necessity to purify unknowns for NMR analysis, it would be desirable to be able to extract structural information from liquid chromatography-mass spectrometry data directly. However, mass spectral libraries for metabolomics are scarce, and no libraries exist for the lignome. Therefore, elucidating the gas-phase fragmentation behavior of the major bonding types encountered in lignome-associated molecules would considerably advance the systematic characterization of the lignome. By comparative MS" analysis of a series of molecules belonging to the beta-aryl ether, benzodioxane, phenylcoumaran, and resinol groups, we succeeded in annotating typical fragmentations for each of these bonding structures as well as fragmentations that enabled the identification of the aromatic units involved in each bonding structure. Consequently, this work lays the foundation for a detailed characterization of the lignome in different plant species, mutants, and transgenics and for the MS-based sequencing of lignin oligomers and (neo)lignans. |
| Databáze: | OpenAIRE |
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