Development of diagnostic antibodies against immunoglobulin heavy chain variable region for heavy chain amyloidosis (AH amyloidosis)

Autor:	Masahide Yazaki, Fuyuki Kametani, Yoshiki Sekijima, Nagaaki Katoh, Tsuneaki Yoshinaga, Keiichi Higuchi, Mayuko Nakagawa
Rok vydání:	2021
Předmět:	0301 basic medicine Pathology medicine.medical_specialty Immunoglobulin Heavy Chain Variable Region Immunologic Tests Antibodies Pathology and Forensic Medicine 03 medical and health sciences 0302 clinical medicine Humans Medicine Heavy chain biology business.industry Amyloidosis Diagnostic marker General Medicine medicine.disease Serum samples Immunohistochemistry 030104 developmental biology 030220 oncology & carcinogenesis biology.protein Immunoglobulin heavy chain Antibody Immunoglobulin Heavy Chains business
Zdroj:	Pathology International. 71:245-254
ISSN:	1440-1827 1320-5463
DOI:	10.1111/pin.13081
Popis:	It is difficult to diagnose immunoglobulin heavy chain amyloidosis (AH amyloidosis) without proteomic analysis due to no useful diagnostic antibodies. The aim of this study was to develop diagnostic antibodies available to immunohistochemistry and immunoblotting. Two rabbit anti-heavy chain variable region antibodies were generated and evaluated in immunohistochemical studies performed on 11 AH amyloidosis patients and 64 patients with other systemic amyloidoses. Additionally, immunoblotting was performed using extracted amyloid protein from one patient and serum samples from two patients with AH amyloidosis. Immunohistochemical analysis generated a positive outcome in 10 of 11 AH amyloidosis patients (sensitivity 90.9%). While positive staining was also observed in 9 of 64 non-AH amyloidosis patients (specificity 85.9%), substitution of the blocking agent reversed the positive reactivity in 5 of 9 patients. Amyloid protein band was clearly detected via immunoblotting analysis, and protein bands with similar molecular weights of amyloid protein were observed in serum samples from patients with AH amyloidosis. The two antibodies may represent a powerful diagnostic tool for AH amyloidosis. In addition, our data revealed the existence of amyloidogenic variable region fragments in the serum of patients, suggesting their potential as diagnostic markers for AH amyloidosis.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cfa1f59d00ea6741b665ffd8e0d3fdb7 https://doi.org/10.1111/pin.13081 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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