Evaluation of the skin phototoxicity of systemically administered pharmaceuticals in Sprague–Dawley rats
| Autor: | Jong Kwon Lee, Ki Sook Park, Eun Ji Kim, Nam Hee Youn, Ki Taek Nam, Jung-Sun Yi, Yejin Cho, Joo Hwan Kim |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Drug
business.industry Health Toxicology and Mutagenesis media_common.quotation_subject Radiation dose Ultraviolet a 010501 environmental sciences Pharmacology Toxicology 030226 pharmacology & pharmacy 01 natural sciences Phototoxicity Study 03 medical and health sciences 0302 clinical medicine In vivo Sprague dawley rats Medicine Original Article Phototoxicity business 0105 earth and related environmental sciences media_common Skin Findings |
| Zdroj: | Toxicol Res |
| Popis: | In vivo phototoxicity testing is important for predicting drug-induced phototoxicity in humans. Currently, there is no internationally validated in vivo test method for the photosafety evaluation of pharmaceuticals. In this study, we evaluated the phototoxicity of systemically administered drugs using SD rats. We first determined the appropriate ultraviolet A (UVA) dose using 8-methoxypsoralen, a well-known phototoxic drug. Compared to lower and higher UVA doses, we found that a UVA dose of 10 J/cm(2) allowed for the detection of phototoxic responses in both a dose- and time-dependent manner. We next performed a phototoxicity study using seven pharmaceutical drugs which included known phototoxic and non-phototoxic drugs using a UVA dose of 10 J/cm(2). In order to improve the accuracy of our assessment, we evaluated both gross skin findings as well as histopathological findings. Using gross skin findings alone resulted in an accuracy of 85.7% which could be increased to 100% accuracy when the gross skin findings were combined with histopathological findings. This study suggests that the inclusion of histopathological findings increases the accuracy of the phototoxicity evaluation of systemically administered drugs in SD rats. In conclusion, we found that for studying drug-induced phytotoxicity, a 10 J/cm(2) UVA dose serves as the optimal radiation dose, and that the inclusion of histopathological findings increases the accuracy of the phototoxicity evaluation of the drugs. |
| Databáze: | OpenAIRE |
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