A human embryonic stem cell-derived clonal progenitor cell line with chondrogenic potential and markers of craniofacial mesenchyme
| Autor: | Walter D. Funk, Shreyasi Das, Qian Wang, Hal Sternberg, Evan Y. Snyder, James T Murai, Karen B. Chapman, C. Thomas Vangsness, Isaac Erickson, Michael D. West |
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| Rok vydání: | 2012 |
| Předmět: |
Embryology
Pathology medicine.medical_specialty Cellular differentiation Sus scrofa Biomedical Engineering Anthraquinones Biology Real-Time Polymerase Chain Reaction Cell Line Mesoderm Osteogenesis Transforming Growth Factor beta Dental pulp stem cells medicine Animals Humans Progenitor cell Dental Pulp Embryonic Stem Cells Stem cell transplantation for articular cartilage repair Cell Proliferation Oligonucleotide Array Sequence Analysis Tissue Engineering Reverse Transcriptase Polymerase Chain Reaction Mesenchymal stem cell Skull Cell Differentiation Mesenchymal Stem Cells Cell biology Clone Cells Rats Endothelial stem cell Gene Expression Regulation Face Collagen Stem cell Chondrogenesis Biomarkers Adult stem cell |
| Zdroj: | Regenerative medicine. 7(4) |
| ISSN: | 1746-076X |
| Popis: | Aims: We screened 100 diverse human embryonic stem-derived progenitor cell lines to identify novel lines with chondrogenic potential. Materials & methods: The 4D20.8 cell line was compared with mesenchymal stem cells and dental pulp stem cells by assessing osteochondral markers using immunohistochemical methods, gene expression microarrays, quantitative real-time PCR and in vivo repair of rat articular condyles. Results: 4D20.8 expressed the site-specific gene markers LHX8 and BARX1 and robustly upregulated chondrocyte markers upon differentiation. Differentiated 4D20.8 cells expressed relatively low levels of COL10A1 and lacked IHH and CD74 expression. Transplantation of 4D20.8 cells into experimentally induced defects in the femoral condyle of athymic rats resulted in cartilage and bone differentiation approximating that of the original tissue architecture. Relatively high COL2A1 and minimal COL10A1 expression occurred during differentiation in HyStem-C hydrogel with TGF-β3 and GDF-5. Conclusion: Human embryonic stem cell-derived embryonic progenitor cell lines may provide a novel means of generating purified site-specific osteochondral progenitor cell lines that are useful in research and therapy.Original submitted 6 March 2012; Revised submitted 5 April 2012; Published online 23 April 2012 |
| Databáze: | OpenAIRE |
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