Recombinant human erythropoietin preconditioning attenuates liver ischemia reperfusion injury through the phosphatidylinositol-3 kinase/AKT/endothelial nitric oxide synthase pathway
| Autor: | Biao Wang, Liyan Chen, Jian Ruan, Xianghong Li, Xin-xin Liao, Kai Wang, Jie Zhou, Wenguang Fu |
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| Rok vydání: | 2012 |
| Předmět: |
Male
medicine.medical_specialty Nitric Oxide Synthase Type III Morpholines Nitric oxide Rats Sprague-Dawley chemistry.chemical_compound Enos Internal medicine medicine Animals Humans Aspartate Aminotransferases Enzyme Inhibitors Ischemic Preconditioning Protein kinase B Erythropoietin biology L-Lactate Dehydrogenase business.industry Alanine Transaminase biology.organism_classification medicine.disease Recombinant Proteins Erythropoietin receptor Rats Disease Models Animal Endocrinology chemistry Alanine transaminase Liver Chromones Reperfusion Injury Immunology biology.protein Surgery Phosphatidylinositol 3-Kinase business Reperfusion injury Proto-Oncogene Proteins c-akt medicine.drug Signal Transduction |
| Zdroj: | The Journal of surgical research. 183(2) |
| ISSN: | 1095-8673 |
| Popis: | Background The exact mechanism by which erythropoietin protects the liver from ischemia reperfusion (I/R) injury is not yet known. In the present study, we examined the role of protein kinase B (PKB/AKT) and endothelial nitric oxide synthase (eNOS) in the protective effect of recombinant human erythropoietin (rHuEPO) on I/R injury of the liver. Materials and methods We used a liver in situ I/R model. One hundred twenty adult male Sprague-Dawley rats were divided randomly into six groups. rHuEPO and (or) LY294002 were injected in the tail vein before the operation, and its effect was assessed by measuring the serum levels of aspartate aminotransferase, alanine aminotransferase, lactate dehydrogenase, nitric oxide (NO), and endothelin-1 (ET-1) and by histologic analysis. The expression of erythropoietin receptor (EPOR) and eNOS was measured by real-time polymerase chain reaction. Total AKT and eNOS and phosphorylated AKT and eNOS were examined by western blot. Results rHuEPO dramatically attenuated the functional and morphologic injuries. The serum levels of alanine aminotransferase and lactate dehydrogenase were significantly decreased, but the amount of NO in the serum was increased in the I/R + rHuEPO group. Accordingly, rHuEPO administration significantly ameliorated the histologic damages at 6 h after reperfusion. rHuEPO significantly stimulated the phosphorylation of AKT and eNOS in the rats after liver I/R. Conclusions The protective effect of rHuEPO in I/R injury is mediated via the activation of the phosphatidylinositol-3 kinase/AKT/eNOS signaling pathway, at least in part, by increasing p-AKT and p-eNOS and leads to the maintenance of an elevated level of NO. |
| Databáze: | OpenAIRE |
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