System Analysis of ROS-Related Genes in the Prognosis, Immune Infiltration, and Drug Sensitivity in Hepatocellular Carcinoma
| Autor: | Liu Liu Zi, Yu Zhou, Chen Chen, Jun Hui Xu, Zhen Dong Qiu, Yong Jun Guan, Xin Zhang, Weixing Wang, Jia Yu, Yi Chao Zhang |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Adult
Male Aging Carcinoma Hepatocellular Adolescent Article Subject Antineoplastic Agents Apoptosis Biology Biochemistry Young Adult Lymphocytes Tumor-Infiltrating medicine Biomarkers Tumor Tumor Cells Cultured Tumor Microenvironment Gene family Humans Gene Aged Cell Proliferation chemistry.chemical_classification Aged 80 and over Gene knockdown Reactive oxygen species QH573-671 Proportional hazards model Gene Expression Profiling Liver Neoplasms Computational Biology Cell Biology General Medicine Middle Aged medicine.disease Prognosis In vitro digestive system diseases Gene Expression Regulation Neoplastic Survival Rate chemistry Drug Resistance Neoplasm Hepatocellular carcinoma Cancer research Female Reactive Oxygen Species Cytology Function (biology) Research Article |
| Zdroj: | Oxidative Medicine and Cellular Longevity, Vol 2021 (2021) Oxidative Medicine and Cellular Longevity |
| ISSN: | 1942-0994 |
| Popis: | Hepatocellular carcinoma (HCC) is an aggressive malignant tumor with a poor prognosis. Reactive oxygen species (ROS) play an important role in tumors; however, the role of ROS-related genes is still unclear in HCC. Therefore, we analyzed the role of ROS-related genes in HCC via bioinformatics methods. Firstly, a prognosis model was constructed using LASSO Cox regression and multivariate analyses. We also investigated the potential function of the ROS-related genes and the correlation with immune infiltration, tumor stemness, and drug sensitivity. ICGC database was used for validation. Secondly, we further analyzed the role of 11 ROS-related genes in HCC. As a member of ROS gene family, the role of STK25 has remained unclear in HCC. We explored the biological function of STK25 using in vitro experiments. The present study was the first to construct a ROS-related prognostic model in HCC. The correlation of ROS-related genes with immune infiltration, tumor stemness, and drug sensitivity was dissected. Furthermore, we demonstrated that STK25 knockdown could increase the proliferation, migration, and invasion capacity of HCC cells. |
| Databáze: | OpenAIRE |
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