Discrimination of oilseed rape volatiles by honey bee: Novel combined gas chromatographic-electrophysiological behavioral assay
| Autor: | Margaret M. Blight, Christine M. Woodcock, Claudine Masson, Lester J. Wadhams, Frédéric Marion-Poll, Minh-Hà Pham-Delègue, V. Kerguelen, M. Le Métayer |
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| Přispěvatelé: | BBSRC Rothamsted Research, Partenaires INRAE, Laboratoire de neurobiologie comparée des invertébrés, Institut National de la Recherche Agronomique (INRA), Centre National de la Recherche Scientifique (CNRS), Unité de phytopharmacie et médiateurs chimiques |
| Rok vydání: | 1994 |
| Předmět: |
0106 biological sciences
HYMENOPTERA Olfaction Biology 01 natural sciences Biochemistry Electroantennography 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Linalool CONDITIONED PROBOSCIS EXTENSION Pollinator [SDV.IDA]Life Sciences [q-bio]/Food engineering [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering COLZA Ecology Evolution Behavior and Systematics Chromatography Apidae OLFACTORY DISCRIMINATION APIS MELLIFERA General Medicine Honey bee biology.organism_classification Apoidea INSECTE 010602 entomology Aculeata chemistry GAS CHROMATOGRAPHY APIDAE HONEY BEE 030217 neurology & neurosurgery ELECTROANTENNOGRAM |
| Zdroj: | Journal of Chemical Ecology Journal of Chemical Ecology, Springer Verlag, 1994, 20 (12), pp.3221-3231. ⟨10.1007/BF02033722⟩ Scopus-Elsevier |
| ISSN: | 1573-1561 0098-0331 |
| DOI: | 10.1007/bf02033722 |
| Popis: | International audience; A novel technique for the simultaneous monitoring of electroan-tennogram (EAG) and conditioned proboscis extension (CPE) responses of honey bees to the effluent from a gas chromatograph (GC) was developed to locate biologically active components in blends of plant volatiles and to investigate odor recognition at the peripheral and behavioral levels. A six-component mixture, comprising compounds previously identified as oilseed rape floral volatiles, was used as the stimulus. Standard CPE and EAG recordings were done as a reference. EAG responses were elicited from unconditioned bees by all the components presented either in the coupled or the standard mode. Conditioned bees gave larger EAG responses than unconditioned bees, suggesting that antennal sensitivity is enhanced by conditioning. At the behavioral level, in both the standard and the coupled modes, only conditioned bees showed the proboscis extension response, with the majority of individuals responding to linalool, 2-phenylethanol, and benzyl alcohol. |
| Databáze: | OpenAIRE |
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