Occurrence of Mycobacterium bovis and non-tuberculous mycobacteria (NTM) in raw and pasteurized milk in the northwestern region of Paraná, Brazil
| Autor: | Sônia Aparecida Sgarioni, Clarice Queico Fujimura Leite, Katiany Rizzieri Caleffi-Ferracioli, Vera Lúcia Dias Siqueira, Karina Andrade de Prince, Dirceu Vedovello Filho, Rosario Dominguez Crespo Hirata, Rosilene Fressatti Cardoso, Mario Hiroyuki Hirata, Sergio Roberto de Andrade Leite |
|---|---|
| Přispěvatelé: | Universidade Estadual de Maringá (UEM), Universidade de São Paulo (USP), Universidade Estadual Paulista (Unesp), Secretaria de Saúde do Estado do Paraná |
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Veterinary medicine
lcsh:QR1-502 Pasteurization Microbiology lcsh:Microbiology law.invention Mycobacterium non-tuberculous mycobacteria fluids and secretions law Raw Foods Medicine Animals mycolic acids analysis Mycobacterium bovis Bacteriological Techniques milk biology business.industry food and beverages Nontuberculous Mycobacteria biology.organism_classification bacterial infections and mycoses Acid-fast Food Microbiology Polymorphism analysis PCR-PRA business Brazil Research Paper |
| Zdroj: | Brazilian Journal of Microbiology, Volume: 45, Issue: 2, Pages: 707-711, Published: JUN 2014 Brazilian Journal of Microbiology, Vol 45, Iss 2, Pp 707-711 (2014) SciELO Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP Brazilian Journal of Microbiology v.45 n.2 2014 Brazilian Journal of Microbiology Sociedade Brasileira de Microbiologia (SBM) instacron:SBM |
| ISSN: | 1517-8382 |
| Popis: | Made available in DSpace on 2015-02-02T12:39:27Z (GMT). No. of bitstreams: 0 Previous issue date: 2014-06-01Bitstream added on 2015-02-02T13:08:32Z : No. of bitstreams: 1 S1517-83822014000200046.pdf: 445060 bytes, checksum: 2de9a82297c238421184d1c69bfafc25 (MD5) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Milk is widely consumed in Brazil and can be the vehicle of agent transmission. In this study, was evaluated the occurrence of Mycobacterium bovis and non-tuberculous mycobacteria (NTM) in raw and pasteurized milk consumed in the northwestern region of Paraná, Brazil. Fifty-two milk samples (20 pasteurized and 32 raw) from dairy farms near the municipality of Maringa, Parana State, Brazil were collected. Milk samples were decontaminated using 5% oxalic acid method and cultured on Lowenstein-Jensen and Stonebrink media at 35 °C and 30 °C, with and without 5-10% CO2. Mycobacteria isolates were identified by morphological features, PCR-Restriction Fragment Length Polymorphism Analysis (PCR-PRA) and Mycolic acids analysis. Thirteen (25%) raw and 2 (4%) pasteurized milk samples were positive for acid fast bacilli growth. Nine different species of NTM were isolated (M. nonchromogenicum, M. peregrinum, M. smegmatis, M. neoaurum, M. fortuitum, M. chelonae, M. flavescens, M. kansasii and M. scrofulaceum). M. bovis was not detected. Raw and pasteurized milk may be considered one source for NTM human infection. The paper reinforces the need for intensification of measures in order to avoid the milk contamination and consequently prevent diseases in the south of Brazil. Universidade Estadual de Maringá Departamento de Análises Clínicas e Biomedicina Universidade de São Paulo Departamento de Análises Clínicas e Toxicológicas Universidade Estadual Paulista Faculdade de Ciências Farmacêutica Universidade Estadual Paulista Instituto de Química Secretaria de Saúde do Estado do Paraná Universidade Estadual Paulista Faculdade de Ciências Farmacêutica Universidade Estadual Paulista Instituto de Química |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|