Conformational flexibility in the enterovirus RNA replication platform
| Autor: | Jordan E. Burke, Steven M. Pascal, Komala Ponniah, Samuel E. Butcher, Kai Cai, Gabriel Cornilescu, Meghan S. Warden |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Gene Expression Regulation
Viral Models Molecular Flexibility (anatomy) Magnetic Resonance Spectroscopy Transcription Genetic Biology medicine.disease_cause Virus Replication Genome Article Protein–protein interaction 03 medical and health sciences Structure-Activity Relationship Replication (statistics) medicine Magnesium Molecular Biology 030304 developmental biology Enterovirus 0303 health sciences Small-angle X-ray scattering 030302 biochemistry & molecular biology RNA Nuclear magnetic resonance spectroscopy medicine.anatomical_structure Biophysics Nucleic Acid Conformation RNA Viral |
| Popis: | A presumed RNA cloverleaf (5′CL), located at the 5′-most end of the noncoding region of the enterovirus genome, is the primary established site for initiation of genomic replication. Stem–loop B (SLB) and stem–loop D (SLD), the two largest stem–loops within the 5′CL, serve as recognition sites for protein interactions that are essential for replication. Here we present the solution structure of rhinovirus serotype 14 5′CL using a combination of nuclear magnetic resonance spectroscopy and small-angle X-ray scattering. In the absence of magnesium, the structure adopts an open, somewhat extended conformation. In the presence of magnesium, the structure compacts, bringing SLB and SLD into close contact, a geometry that creates an extensive accessible major groove surface, and permits interaction between the proteins that target each stem–loop. |
| Databáze: | OpenAIRE |
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