Nitric oxide is involved in methane-induced adventitious root formation in cucumber
| Autor: | Weiti Cui, Fang Qi, Zhixin Xiang, Ren Wang, Wenbiao Shen, Ninghai Kou, Dan Zhu, Daokun Xu |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Nitroprusside
0106 biological sciences 0301 basic medicine Physiology Cell Organogenesis Endogeny Plant Science Biology Nitric Oxide Plant Roots 01 natural sciences Nitric oxide 03 medical and health sciences chemistry.chemical_compound Plant Growth Regulators Gene Expression Regulation Plant Botany Genetics medicine Plant Proteins Regulation of gene expression Indoleacetic Acids Cell Biology General Medicine NONOate Cell biology 030104 developmental biology medicine.anatomical_structure chemistry Sodium nitroprusside Cucumis sativus Signal transduction Methane Biomarkers Signal Transduction 010606 plant biology & botany medicine.drug |
| Zdroj: | Physiologia Plantarum. 159:366-377 |
| ISSN: | 0031-9317 |
| DOI: | 10.1111/ppl.12531 |
| Popis: | Our previous studies revealed that methane (CH4 ) induces adventitious rooting in cucumber. However, the corresponding molecular mechanism is still elusive. In this work, we discovered that CH4 triggered the accumulation of nitric oxide (NO) and thereafter cucumber adventitious rooting, mimicking the inducing effects of sodium nitroprusside (SNP) and NONOate (two NO-releasing compounds). Above mentioned responses were sensitive to NO scavenger(s), showing that the accumulation of NO and adventitious root development were respectively impaired. Inhibitor test and biochemical analysis suggested that endogenous NO mainly produced by mammalian NO synthase-like enzyme and diamine oxidases (DAO), might be required for adventitious root formation elicited by CH4 . Molecular evidence confirmed that CH4 -mediated induction of several marker genes responsible for adventitious root development, including CsDNAJ-1, CsCDPK1, CsCDPK5, cell division-related gene CsCDC6, and two auxin signaling genes, CsAux22D-like and CsAux22B-like, was casually dependent on NO signaling. The possible involvement of S-nitrosylation during the mentioned CH4 responses was preliminarily illustrated. Taken together, through pharmacological, anatomical and molecular approaches, it is suggested that NO might be involved in CH4 -induced cucumber adventitious rooting, and CH4 -eliciated NO-targeted proteins might be partially modulated at transcriptional and post-translational levels. Our work may increase the understanding of the mechanisms underlying CH4 -elicited root organogenesis in higher plants. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text