Adaptation of Drosophila enzymes to temperature—V. Heat shock effect on the malate dehydrogenase of Drosophila melanogaster
| Autor: | S. N. Alahiotis, G. Goulielmos, G. Kilias |
|---|---|
| Rok vydání: | 1986 |
| Předmět: |
chemistry.chemical_classification
Hot Temperature Strain (chemistry) Physiology General Medicine Biology biology.organism_classification Biochemistry Malate dehydrogenase Kinetics Drosophila melanogaster Enzyme chemistry Malate Dehydrogenase Drosophilidae Melanogaster Animals Thermodynamics Specific activity Molecular Biology Thermostability |
| Zdroj: | Comparative Biochemistry and Physiology Part B: Comparative Biochemistry. 85:229-234 |
| ISSN: | 0305-0491 |
| DOI: | 10.1016/0305-0491(86)90247-6 |
| Popis: | 1. 1. Drosophila melanogaster cMdh allozymic variants ( Mdh F , Mdh F/S , Mdh S ) were subjected to heat shock ( 33° C /30 min →40° C /30 min ). 2. 2. This stress increases differentially MDH specific activity, with the cMdh F strain showing greater response as compared with the cMdh S one; heterozygotes exhibited generally intermediate values. 3. 3. Correlative differences were also revealed for some catalytic properties ( V max , V max K max ratio, thermostability) of the cMDH; this is not true for the mMDH. 4. 4. The catalytic behavior of the enzyme is correlated with the differential survival of the cMdh variants, with the cMdh F showing again higher survival than the cMdh S one, a fact which seems to contribute to temperature adaptation of D. melanogaster . |
| Databáze: | OpenAIRE |
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