Stability of argECBH messenger RNA under arginine excess or restriction
| Autor: | William L. McLellan, Henry L. Vogel |
|---|---|
| Rok vydání: | 1973 |
| Předmět: |
Ornithine
Arginine Biophysics Acetates Biology Tritium medicine.disease_cause Biochemistry chemistry.chemical_compound Gene cluster Escherichia coli medicine RNA Messenger Uridine Molecular Biology Messenger RNA Strain (chemistry) Nucleic Acid Hybridization Cell Biology Molecular biology Kinetics chemistry Rifampin After treatment Half-Life |
| Zdroj: | Biochemical and Biophysical Research Communications. 55:1385-1389 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/s0006-291x(73)80047-6 |
| Popis: | Summary The decay of mRNA's corresponding to the argECBH gene cluster of Escherichia coli has been studied by RNA-DNA hybridization methods. In the argA−argR+ strain 39A-23R3, the mRNA's were labeled with tritiated uridine either during message accumulation by arginine starvation or during growth under conditions derepressive for the arginine system. The decay of the messages was followed, in the presence of a repressive ( l -arginine hydrochloride) or a restrictive (α- N -acetyl- l -ornithine) arginine source, by measuring the disappearance of radioactive label after a chase with cold uridine or after treatment of the cells with rifampicin or after both. Accumulated argECBH messages show half-lives of 1.5 and 4.3 min for arginine excess and arginine restriction, respectively. Under conditions of growth, i. e., under physiological conditions, the corresponding half-lives are 1.6 and 6.6 min. |
| Databáze: | OpenAIRE |
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