Characterization of scallop midgut gland certified reference material for quantification of diarrhetic shellfish toxins
| Autor: | Toshiyuki Suzuki, Ayano Miyamoto, Migaku Kawaguchi, Taichi Yamazaki, Shinsuke Inagaki, Takashi Yarita, Akiko Takatsu, Hajime Uchida |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Diarrhea
01 natural sciences Analytical Chemistry chemistry.chemical_compound 0404 agricultural biotechnology Tandem Mass Spectrometry Liquid chromatography–mass spectrometry Okadaic Acid medicine Animals Humans Shellfish Poisoning Shellfish Pyrans Chromatography 010401 analytical chemistry Midgut 04 agricultural and veterinary sciences General Medicine Okadaic acid Reference Standards medicine.disease 040401 food science 0104 chemical sciences Shellfish poisoning Intestines Pectinidae Certified reference materials chemistry Standard addition Calibration Scallop Marine Toxins Chromatography Liquid Food Science |
| Zdroj: | Food Chemistry. 298:125011 |
| ISSN: | 0308-8146 |
| Popis: | A scallop midgut gland certified reference material, NMIJ CRM 7520-a, was developed for validation and quality assurance during the inspection of shellfish for diarrhetic shellfish toxins. The candidate material was prepared by using naturally-toxic and nontoxic boiled midgut glands spiked with okadaic acid (OA). The homogeneity and stability of the material were found to be appropriate. For the characterization of OA and dinophysistoxin-1 (DTX1), nine participants were involved in a co-laboratory study based on the Japanese Official Testing Method, where the compounds were assayed by liquid chromatography-tandem mass spectrometry following alkaline hydrolysis. The analytical values were obtained by the standard addition method with a standard spiking solution calibrated using the standard-solution certified reference materials OA and DTX1. The certified concentrations with expanded uncertainties (coverage factor k = 2, approximate 95% confidence interval) were determined to be (0.205 ± 0.061) mg/kg for OA and (0.45 ± 0.11) mg/kg for DTX1. |
| Databáze: | OpenAIRE |
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