The effect of maternal HMB supplementation on bone mechanical and geometrical properties, as well as histomorphometry and immunolocalization of VEGF, TIMP2, MMP13, BMP2 in the bone and cartilage tissue of the humerus of their newborn piglets
| Autor: | Piotr Dobrowolski, Jaromir Jarecki, Tomasz Blicharski, Siemowit Muszyński, Lucio C. Rovati, Agnieszka Tomczyk-Warunek, Ewa Tomaszewska |
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| Rok vydání: | 2020 |
| Předmět: |
Male
Vascular Endothelial Growth Factor A Cytoplasm Swine Maternal Health VEGF receptors Protein Expression Bone Morphogenetic Protein 2 Physiology Biochemistry 0403 veterinary science Pregnancy Medicine and Health Sciences Medicine Growth Plate skin and connective tissue diseases Musculoskeletal System Mammals 0303 health sciences Multidisciplinary biology Obstetrics and Gynecology Eukaryota 04 agricultural and veterinary sciences Breed medicine.anatomical_structure Connective Tissue Maternal Exposure Vertebrates Animal Nutritional Physiological Phenomena Female Anatomy Cellular Structures and Organelles Research Article 040301 veterinary sciences Offspring Science Research and Analysis Methods Bone morphogenetic protein 2 Bone and Bones 03 medical and health sciences Matrix Metalloproteinase 13 Gene Expression and Vector Techniques Valerates Animals Humerus Bone Molecular Biology Techniques Molecular Biology Skeleton 030304 developmental biology Molecular Biology Assays and Analysis Techniques Tissue Inhibitor of Metalloproteinase-2 Growth cartilage business.industry Cartilage Organisms Biology and Life Sciences Proteins Cell Biology medicine.disease Animal Feed Diet Biological Tissue Animals Newborn Amniotes Dietary Supplements biology.protein Women's Health Poland business Collagens Zoology |
| Zdroj: | PLoS ONE PLoS ONE, Vol 16, Iss 2, p e0240642 (2021) |
| Popis: | The presented experiment focuses on assessing the impact of HMB (hydroxy-β-methobutyrate) supplementation of mothers during pregnancy on the development of the skeletal system of their offspring. For this purpose, an experiment was carried out on 12 clinically healthy sows of the Great White Poland breed, which were divided randomly into two groups the control and the HMB group. All animals were kept under standard conditions and received the same feed for pregnant females. In contrast, females from the HMB group between 70 and 90 days were supplemented with 3-hydroxy-3-methylbutyle in the amount of 0.2g/kg b.w/day. Immediately after birth, the piglets were also divided into groups based on: sex, and presence or lack HMB supplementation, and subsequently were euthanized and humerus bones from all piglets were collected.Mother’s HMB supplementation during pregnancy affected the multiple index of their offspring. The higher humerus mass and length was observed with the greater effect in males. Maternal supplementation also influenced on the geometrical and mechanical properties of the humerus as in the case of mass, this effect was higher in males. Also, the collagen structure of the compacted and trabecular bone changed under the HMB addition. Maternal supplementation also affected the expression of selected proteins in growth cartilage and trabecular bone. The obtained results show that the administration to the mother during pregnancy by the HMB significantly affects the development of the humerus in many ways. The obtained results also confirm the utility of such experiments in understanding of the importance of the pregnancy diet as an develop and adaptable factor of offspring organisms and are the base for further research in that area as well as in the protein markers expression area. |
| Databáze: | OpenAIRE |
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