One-Step Preparation of Chitosan-Coated Cationic Liposomes by an Improved Supercritical Reverse-Phase Evaporation Method
| Autor: | Toshihiro Goto, Tomohiro Imura, Shimomura Takeshi, Katsuto Otake, Masahiko Abe, Satoshi Yoda, Hideki Sakai, Takeshi Furuya, Yoshihiro Takebayashi |
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| Rok vydání: | 2006 |
| Předmět: |
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2-Dipalmitoylphosphatidylcholine Evaporation In Vitro Techniques Chitosan chemistry.chemical_compound Drug Delivery Systems Cations Methods Electrochemistry Freeze Fracturing General Materials Science Cationic liposome Particle Size Spectroscopy Carbonic acid Liposome Chromatography Chemistry Surfaces and Interfaces Condensed Matter Physics Supercritical fluid Chemical engineering Liposomes Particle size Dispersion (chemistry) |
| Zdroj: | Langmuir. 22:4054-4059 |
| ISSN: | 1520-5827 0743-7463 |
| Popis: | High-pressure carbon dioxide in contact with water dissolves to form carbonic acid, causing a decrease in pH. By use of these characteristics of a CO2/H2O biphasic system, chitosan-coated cationic liposomes of l-alpha-dipalmitoylphosphatidylcholine were successfully prepared by an improved supercritical reverse-phase evaporation (ISCRPE) method. Liposome-chitosan complexes carrying a positive charge were prepared in a single-step procedure without the use of acid or organic solvent, including ethanol. The maximum trapping efficiency of liposomes prepared by the ISCRPE method was 17%, with or without the addition of chitosan, compared to only 2% for liposomes prepared by the Bangham method. Furthermore, the liposomal dispersion was stable at room temperature in a sealed tube for over 30 days. |
| Databáze: | OpenAIRE |
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