A reduction in DNA damage in neural tissue and peripheral blood of old mice treated with caffeine
| Autor: | Larissa Letieli Toniazzo de Abreu, Michelle Lima Garcez, Taís Helena Tavares, Adriani Paganini Damiani, Carina Rodrigues Boeck, Vanessa Moraes de Andrade |
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| Rok vydání: | 2017 |
| Předmět: |
Male
0301 basic medicine medicine.medical_specialty DNA damage Health Toxicology and Mutagenesis Biology Hippocampal formation Toxicology medicine.disease_cause Hippocampus Mice 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Caffeine Internal medicine medicine Animals Nerve Tissue Young adult Micronucleus Tests Age Factors Comet assay Blood 030104 developmental biology Endocrinology chemistry Immunology Micronucleus test Central Nervous System Stimulants Comet Assay Micronucleus 030217 neurology & neurosurgery Genotoxicity DNA Damage |
| Zdroj: | Journal of Toxicology and Environmental Health, Part A. 80:621-629 |
| ISSN: | 1087-2620 1528-7394 |
| DOI: | 10.1080/15287394.2017.1286901 |
| Popis: | Studies on caffeine consumption have shown a negative correlation with development of some diseases with subsequent beneficial manifestations. Our aim was to assess the effects of caffeine on peripheral blood and neural tissue DNA in young adult and aged mice. Male Swiss mice (age 2-3 or 16-18 months, respectively) were treated with a caffeine solution (0.3 g/l) for 4 weeks, while controls received water. After the treatments, blood and hippocampal cells (for a comet assay) and femurs (for a micronucleus [MN] test) were collected. The comet assay of peripheral blood and hippocampal cells demonstrated no significant differences between caffeine-treated and control young adult mice in terms of DNA damage index (DI) and frequency. In contrast, when comparing young adult with aged animals, significant differences were observed in DNA damage in blood and hippocampal cells. The differences between aged animals (with or without caffeine) consisted of a significant decrease in DNA DI in the group that received caffeine. In the MN test, an increase in frequency of micronucleated polychromatic (PCE) erythrocytes was noted in aged animals that received water compared to young adult mice. In addition, comparing treated with control aged murine groups, a decrease in frequency of MN was found in PCE erythrocytes of caffeine-treated mice. Chronic caffeine consumption was neither genotoxic nor mutagenic at the dose tested; however, it appears that caffeine actually protected mice from genotoxicity and mutagenicity, consequences attributed to aging. |
| Databáze: | OpenAIRE |
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