Intracellular hepatitis B virus increases hepatic cholesterol deposition in alcoholic fatty liver via hepatitis B core protein
| Autor: | Qin Ning, Xinxin Weng, Yaqi Wang, Pei-Jer Chen, Xiaoping Luo, Ting Wu, Hongwu Wang, Xiaojing Wang, Danqing Hu |
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| Rok vydání: | 2018 |
| Předmět: |
Male
0301 basic medicine medicine.medical_specialty Alcoholic liver disease Cirrhosis Mice Inbred Strains QD415-436 medicine.disease_cause Biochemistry Mice 03 medical and health sciences chemistry.chemical_compound Endocrinology Internal medicine Tumor Cells Cultured medicine Animals Humans Research Articles Hepatitis B virus business.industry Cholesterol Viral Core Proteins cholesterol virus diseases Hep G2 Cells Cell Biology Hepatitis B medicine.disease digestive system diseases Disease Models Animal HBx 030104 developmental biology Liver chemistry Hepatocellular carcinoma lipids (amino acids peptides and proteins) Alcoholic fatty liver business hepatitis B virus Fatty Liver Alcoholic alcoholic liver disease |
| Zdroj: | Journal of Lipid Research, Vol 59, Iss 1, Pp 58-68 (2018) Journal of Lipid Research |
| ISSN: | 0022-2275 |
| Popis: | Hepatitis B virus (HBV) infection is a prevalent infectious disease with serious outcomes like chronic and acute hepatitis, cirrhosis, and hepatocellular carcinoma. However, the metabolic alteration by HBV is rarely taken into consideration. With the high prevalence of alcohol consumption and chronic HBV infection, their overlap is assumed to be an increasing latent hazard; although the extent has not been calculated. Moreover, the impact of chronic alcohol consumption combined with HBV on cholesterol metabolism is unknown. Six-week-old male FVB/Ncrl mice were hydrodynamically injected with a pGEM-4Z-1.3HBV vector and then fed an ethanol diet for 6 weeks. Serum biomarkers and liver histology, liver cholesterol levels, and cholesterol metabolism-related molecules were measured. In vitro assays with HBx, hepatitis B surface (HBs), or hepatitis B core (HBc) protein expression in HepG2 cells costimulated with ethanol were conducted to assess the cholesterol metabolism. HBV expression synergistically increased cholesterol deposition in the setting of alcoholic fatty liver. The increase of intrahepatic cholesterol was due to metabolic alteration in cholesterol metabolism, including increased cholesterol synthesis, decreased cholesterol degradation, and impaired cholesterol uptake. Overexpression of HBV component HBc, but not HBs or HBx, selectively promoted the hepatocellular cholesterol level. |
| Databáze: | OpenAIRE |
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