Positive Regulation of Lyn Kinase by CD148 Is Required for B Cell Receptor Signaling in B1 but Not B2 B Cells
| Autor: | Jing W. Zhu, Katarzyna M. Skrzypczynska, Arthur Weiss |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Enzyme-Linked Immunospot Assay Cell Regulator Receptor-Like Protein Tyrosine Phosphatases Lymphocyte Activation Mice 0302 clinical medicine hemic and lymphatic diseases Receptors Immunology and Allergy Mice Knockout B1 B cell Kinase DEP-1 Receptor-Like Protein Tyrosine Phosphatases Class 3 breakpoint cluster region BCR Flow Cytometry Cell biology src-Family Kinases Infectious Diseases medicine.anatomical_structure Antigen 030220 oncology & carcinogenesis CD148 Signal Transduction Knockout 1.1 Normal biological development and functioning Immunology B-cell receptor B-Lymphocyte Subsets Receptors Antigen B-Cell Enzyme-Linked Immunosorbent Assay Biology T-cell-independent Article Ptprj 03 medical and health sciences Underpinning research LYN medicine Animals Lyn B cell Inflammatory and immune system B-Cell Class 3 030104 developmental biology Pneumovax 23 Cancer research |
| Zdroj: | Immunity, vol 45, iss 6 |
| ISSN: | 1074-7613 |
| DOI: | 10.1016/j.immuni.2016.10.013 |
| Popis: | B1 and B2 B cells differ in their ability to respond to T-cell-independent (TI) antigens. Here we report that the Src-family kinase (SFK) regulator CD148 has a unique and critical role in the initiation of B1 but not B2 cell antigen receptor signaling. CD148 loss-of-function mice were found to have defective B1 B-cell-mediated antibody responses against the T-cell-independent antigens NP-ficoll and Pneumovax 23 and had impaired selection of the B1 B cell receptor (BCR) repertoire. These deficiencies were associated with a decreased ability of B1 B cells to induce BCR signaling downstream of the SFK Lyn. Notably, Lyn appeared to be selectively regulated by CD148 and loss of this SFK resulted in opposite signaling phenotypes in B1 and B2 B cells. These findings reveal that the function and regulation of Lyn during B1 cell BCR signaling is distinct from other B cell subsets. |
| Databáze: | OpenAIRE |
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