Identification of Leishmania selenoproteins and SECIS element
| Autor: | Alexandre Cassago, Juan D. Alfonzo, Angela K. Cruz, Kirk W. Gaston, E.L. Prieto, Otavio Henrique Thiemann, E. M. Rodrigues, M.J. Berry, M.P. Iribar |
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| Rok vydání: | 2006 |
| Předmět: |
Molecular Sequence Data
Protozoan Proteins chemistry.chemical_compound Sequence Homology Nucleic Acid parasitic diseases Animals Amino Acid Sequence RNA Messenger Selenoproteins Molecular Biology SECIS element Leishmania Genetics chemistry.chemical_classification Base Sequence Sequence Homology Amino Acid Selenocysteine biology Kinetoplastida DNA Protozoan RNA Transfer Amino Acid-Specific biology.organism_classification Open reading frame chemistry Nucleic Acid Conformation Parasitology Selenoprotein Selenocysteine incorporation Leishmania infantum RNA Protozoan |
| Zdroj: | Molecular and Biochemical Parasitology. 149:128-134 |
| ISSN: | 0166-6851 |
| DOI: | 10.1016/j.molbiopara.2006.05.002 |
| Popis: | Selenoproteins result from the incorporation of selenocysteine (Sec-U) at an UGA-stop codon positioned within a gene's open reading frame and directed by selenocysteine insertion sequence (SECIS) elements. Although the selenocysteine incorporation pathway has been identified in a wide range of organisms it has not yet been reported in the Kinetoplastida Leishmania and Trypanosoma. Here we present evidence consistent with the presence of a selenocysteine biosynthetic pathway in Kinetoplastida. These include the existence of SECIS-containing coding sequences in Leishmania major and Leishmania infantum, the incorporation of (75)Se into Leishmania proteins, the occurrence of selenocysteine-tRNA (tRNA (sec) (uca)) in both Leishmania and Trypanosoma and in addition the finding of all genes necessary for selenocysteine synthesis such as SELB, SELD, PSTK and SECp43. As in other eukaryotes, the Kinetoplastids have no identifiable SELA homologue. To our knowledge this is the first report on the identification of selenocysteine insertion machinery in Kinetoplastida, more specifically in Leishmania, at the sequence level. |
| Databáze: | OpenAIRE |
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