GLC determination of 17 alpha-ethynylestriol 3-cyclopentyl ether
| Autor: | Bonnie S. Rutherford, Alan Dinner, Rafik H. Bishara |
|---|---|
| Rok vydání: | 1975 |
| Předmět: |
Peak area
Chloroform Chromatography Chromatography Gas Computers Estriol Pharmaceutical Science Ether Method of analysis Quantitative determination Mass Spectrometry chemistry.chemical_compound Silicone chemistry Estradiol Congeners Norpregnatrienes Methods Quinestrol Estrogenic drug Derivative (chemistry) Tablets |
| Zdroj: | Journal of pharmaceutical sciences. 64(7) |
| ISSN: | 0022-3549 |
| Popis: | A rapid, sensitive, and accurate GLC method of analysis of a new estrogenic drug, 17 alpha-ethynylestriol 3-cyclopentyl ether, was developed. The drug and the internal standard, tetratriacontane, are dissolved in chloroform, and an aliquot is heated with N-trimethylsilylimidazole at 80 degrees for 30 min. The silylated sample is chromatographed using a column packed with 1% methyl vinyl silicone gum on Gas Chrom Q. Quantitation is achieved by computer calculation of the peak area ratios. The observed peak is the 16alpha,17beta-bistrimethylsilyl derivative of the new drug substance. The GLC method was applied to the quantitative determination of the estrogenic compound in a tablet formulation containing 25 mug/tablet. |
| Databáze: | OpenAIRE |
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