The Eicosapentaenoic Acid Metabolite 15-Deoxy-δ12,14-Prostaglandin J3 Increases Adiponectin Secretion by Adipocytes Partly via a PPARγ-Dependent Mechanism
| Autor: | Alain Géloën, Mairobys Socorro, Hubert Vidal, Michel Lagarde, Jennifer Lefils-Lacourtablaise, Michel Guichardant, Nathalie Bernoud-Hubac, Emmanuelle Loizon, Zury Domínguez, Cyrille Debard, Patricia Daira |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Male
lcsh:Medicine Adipose tissue Peroxisome proliferator-activated receptor Biochemistry Mice chemistry.chemical_compound Molecular Cell Biology Adipocytes Macromolecular Structure Analysis Signaling in Cellular Processes Anilides Adiponectin secretion lcsh:Science Epididymis chemistry.chemical_classification Multidisciplinary Prostaglandin D2 Reverse Transcriptase Polymerase Chain Reaction Fatty Acids Lipids Eicosapentaenoic acid Adipose Tissue Eicosapentaenoic Acid Lipid Signaling Medicine lipids (amino acids peptides and proteins) Arachidonic acid Adiponectin Research Article Signal Transduction Polyunsaturated fatty acid medicine.medical_specialty Prostaglandin Biology Gas Chromatography-Mass Spectrometry Lipid Mediators 3T3-L1 Cells Internal medicine Lipid Structure medicine Animals Nutrition lcsh:R Computational Biology Lipid Metabolism Dietary Fats PPAR gamma Metabolism Endocrinology chemistry lcsh:Q |
| Zdroj: | PLoS ONE PLoS ONE, Vol 8, Iss 5, p e63997 (2013) |
| ISSN: | 1932-6203 |
| Popis: | The intake of ω-3 polyunsaturated fatty acids (PUFAs), which are abundant in marine fish meat and oil, has been shown to exert many beneficial effects. The mechanisms behind those effects are numerous, including interference with the arachidonic acid cascade that produces pro-inflammatory eicosanoids, formation of novel bioactive lipid mediators, and change in the pattern of secreted adipocytokines. In our study, we show that eicosapentaenoic acid (EPA) increases secreted adiponectin from 3T3-L1 adipocytes and in plasma of mice as early as 4 days after initiation of an EPA-rich diet. Using 3T3-L1 adipocytes, we report for the first time that 15-deoxy-δ(12,14)-PGJ3 (15d-PGJ3), a product of EPA, also increases the secretion of adiponectin. We demonstrate that the increased adiponectin secretion induced by 15d-PGJ3 is partially peroxisome proliferator-activated receptor-gamma (PPAR-γ)-mediated. Finally, we show that 3T3-L1 adipocytes can synthesize 15d-PGJ3 from EPA. 15d-PGJ3 was also detected in adipose tissue from EPA-fed mice. Thus, these studies provide a novel mechanism(s) for the therapeutic benefits of ω-3 polyunsaturated fatty acids dietary supplementation. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|