Comparison of methods for isolating primary hepatocytes from mini pigs
| Autor: | Li Li, Yujun Shi, Yi Li, Hong Bu, Yujia Wang, Qiong Wu, Ji Bao |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Swine Immunology Transplantation Heterologous Cell Separation law.invention 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Hepatocyte transplantation law Gene expression medicine Animals Humans Cells Cultured Transplantation Bioartificial liver device Albumin Molecular biology Liver Artificial 030104 developmental biology medicine.anatomical_structure chemistry 030220 oncology & carcinogenesis Hepatocyte Urea Collagenase Hepatocytes Porcine hepatocyte Swine Miniature medicine.drug |
| Zdroj: | Xenotransplantation. 23(5) |
| ISSN: | 1399-3089 |
| Popis: | Successful porcine hepatocyte isolation is crucial for the development of bioartificial liver devices and hepatocyte transplantation. Serva collagenase NB grades are formulated collagenases that are suitable for various tissue isolation applications. N-acetylcysteine (NAC) can improve the viability of human hepatocytes. The aim of this study was to compare the effectiveness of two collagenases and effect of NAC on hepatocyte isolation from porcine liver tissue. Porcine hepatocytes were isolated using the perfusion method from Bama mini pigs assigned to the Serva NB 4 group (n=6), the Serva NB 8 group (n=6), or the NB 8+NAC group (n=6). Viability and yield were defined as fresh hepatocytes and their spheroids formation after 24-hour rocker culture in serum-free medium. Metabolic function was assessed by gene expression, albumin, and urea synthesis. All procedures resulted in successful hepatocyte isolation. Cells from the NB 8+NAC group had (97.8±1.9)% viability, which was higher than the NB 8 group with (94.4±2.4)% and the NB 4 group with (94.5±3.2)% (P |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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