Crosstalk between heterotrimeric G protein-coupled signaling pathways and WRKY transcription factors modulating plant responses to suboptimal micronutrient conditions
| Autor: | Richalynn Leong, Shalini Krishnamoorthi, Honzhen Goh, Amy Catherine Sanson, Daisuke Urano, Ting-Ying Wu |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Physiology
G protein Mutant Arabidopsis Plant Science Gene Expression Regulation Plant Heterotrimeric G protein transcription factors Micronutrients G protein signaling Transcription factor biology AcademicSubjects/SCI01210 Arabidopsis Proteins rice GTP-Binding Protein beta Subunits biology.organism_classification Research Papers Heterotrimeric GTP-Binding Proteins WRKY protein domain Cell biology meta-analysis Ion homeostasis Plant—Environment Interactions nutrient stress response Signal transduction |
| Zdroj: | Journal of Experimental Botany |
| ISSN: | 1460-2431 0022-0957 |
| Popis: | Nutrient stresses induce foliar chlorosis and growth defects. Here we propose heterotrimeric G proteins as signaling mediators of various nutrient stresses, through meta-analyses of >20 transcriptomic data sets associated with nutrient stresses or G protein mutations. Systematic comparison of transcriptomic data yielded 104 genes regulated by G protein subunits under common nutrient stresses: 69 genes under Gβ subunit (AGB1) control and 35 genes under Gα subunit (GPA1) control. Quantitative real-time PCR experiments validate that several transcription factors and metal transporters changed in expression level under suboptimal iron, zinc, and/or copper concentrations, while being misregulated in the Arabidopsis Gβ-null (agb1) mutant. The agb1 mutant had altered metal ion profiles and exhibited severe growth arrest under zinc stress, and aberrant root waving under iron and zinc stresses, while the Gα-null mutation attenuated leaf chlorosis under iron deficiency in both Arabidopsis and rice. Our transcriptional network analysis inferred computationally that WRKY-family transcription factors mediate the AGB1-dependent nutrient responses. As corroborating evidence of our inference, ectopic expression of WRKY25 or WRKY33 rescued the zinc stress phenotypes and the expression of zinc transporters in the agb1-2 background. These results, together with Gene Ontology analyses, suggest two contrasting roles for G protein-coupled signaling pathways in micronutrient stress responses: one enhancing general stress tolerance and the other modulating ion homeostasis through WRKY transcriptional regulatory networks. In addition, tolerance to iron stress in the rice Gα mutant provides an inroad to improve nutrient stress tolerance of agricultural crops by manipulating G protein signaling. Transcriptomic gene regulatory network analysis demonstrates two contrasting roles of the G proteins Gα and Gβ in micronutrient stress responses and ion homeostasis. |
| Databáze: | OpenAIRE |
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