Inkjet printed and 'doctor blade' TiO2 photodetectors for DNA biosensors
Autor: | Hugo Águas, Rohan Senadeera, L.B. Silva, Pedro V. Baptista, I. Bernacka-Wojcik, Gonçalo Doria, Elvira Fortunato, Paweł Wójcik, Rodrigo Martins |
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Rok vydání: | 2010 |
Předmět: |
DNA
Bacterial Materials science Fabrication Biomedical Engineering Biophysics Nanoparticle Nanoprobe Photodetector Nanotechnology Biosensing Techniques Sensitivity and Specificity Photometry Electrochemistry Computer Peripherals Inkjet printing Oligonucleotide Array Sequence Analysis Titanium Reproducibility of Results Equipment Design Mycobacterium tuberculosis General Medicine Equipment Failure Analysis Dna detection Colloidal gold Biosensor Biotechnology |
Zdroj: | Biosensors and Bioelectronics. 25:1229-1234 |
ISSN: | 0956-5663 |
DOI: | 10.1016/j.bios.2009.09.027 |
Popis: | A dye sensitized TiO(2) photodetector has been integrated with a DNA detection method based on non-cross-linking hybridization of DNA-functionalized gold nanoparticles, resulting in a disposable colorimetric biosensor. We present a new approach for the fabrication of dye sensitized TiO(2) photodetectors by an inkjet printing technique-a non-contact digital, additive, no mask and no vacuum patterning method, ideal for cost efficient mass production. The developed biosensor was compared against a dye sensitized photodetector fabricated by the traditional "doctor blade" method. Detection of gold nanoparticle aggregation was possible for concentrations as low as 1.0 nM for the "doctor blade" system, and 1.5 nM for the inkjet printed photodetector. The demonstrated sensitivity limits of developed biosensors are comparable to those of spectrophotometric techniques (1.0 nM). Our results show that a difference higher than 17% by traditional photodetector and 6% by inkjet printed in the photoresponses for the complementary and non-complementary gold nanoprobe assays could be attained for a specific DNA sequence from Mycobacterium tuberculosis, the etiologic agent of human tuberculosis. The decrease of costs associated with molecular diagnostic provided by a platform such as the one presented here may prove of paramount importance in developing countries. |
Databáze: | OpenAIRE |
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