Cyclic enterobacterial common antigens fromEscherichia coliO157 as microbe-associated molecular patterns
| Autor: | Velichka Pavlova, Elena Nikolova, Tsvetelina Paunova-Krasteva, Radka Ivanova, Stoyanka Stoitsova, Antonio Molinaro, Cristina De Castro |
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| Přispěvatelé: | Tsvetelina S., Paunova Krasteva, Velichka A., Pavlova, DE CASTRO, Cristina, Radka M., Ivanova, Molinaro, Antonio, Elena B., Nikolova, Stoyanka R., Stoitsova |
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Lipopolysaccharides
Enterobacterial common antigen beta-Glucans Free Radicals Pentamer Neutrophils Phagocytosis Immunology Enzyme-Linked Immunosorbent Assay Biology medicine.disease_cause Escherichia coli O157 Ligands Applied Microbiology and Biotechnology Microbiology Mannose-Binding Lectin Tetramer Antigen Genetics medicine Animals Humans Molecular Biology Escherichia coli Antigens Bacterial Phagocytes Immune Sera General Medicine Microspheres Biochemistry Host-Pathogen Interactions |
| Popis: | In a previous study, we described 2 forms of cyclic enterobacterial common antigen (ECACYC), a tetramer and a pentamer, from Escherichia coli O157. ECACYCis present in several representatives of the Enterobacteriaceae. To date, functional studies on ECACYCare sparse. Cyclic oligosaccharides in other bacteria, like the cyclic β-glucans in Rhizobiaceae, represent microbe-associated molecular patterns involved in host–bacteria interaction. This observation determined the aim of the present study: to test whether the tetrameric and pentameric ECACYCfrom E. coli O157 can be recognised by host humoral and cellular mechanisms. ELISA tests designed to compare the 2 ECACYCwith the O157 lipopolysaccharide showed that both ECACYCwere not recognised by polyclonal anti-O157 serum but were good ligands for mannan-binding lectin. The lectin had a higher affinity for the tetramer than the pentamer. ECACYCdeposited more C3b than did the lipopolysaccharide. To examine the interactions with human circulating neutrophils, the antigens were loaded onto fluorescent latex beads and applied in a phagocytosis experiment. Spheres coated with the 2 ECACYCoccasionally adhered to phagocyte surfaces but, unlike O157-loaded spheres, failed to induce free-radical release. The results show that the 2 ECACYCrepresent microbe-associated molecular patterns recognised by host humoral non-self-recognition mechanisms. |
| Databáze: | OpenAIRE |
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