Novel gateway binary vectors for rapid tripartite DNA assembly and promoter analysis with various reporters and tags in the liverwort Marchantia polymorpha
| Autor: | Ryuichi Nishihama, Kazumi Hikino, Katsuyuki T. Yamato, Tsuyoshi Nakagawa, Sakiko Ishida, Takayuki Kohchi, Shoji Mano, Mikio Nishimura, Maki Kondo |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine Time Factors Molecular biology lcsh:Medicine Genetically modified crops Plant Science 01 natural sciences Genetically Modified Plants Marchantia polymorpha Plasmid Genes Reporter Arabidopsis thaliana Coding region Vector (molecular biology) Promoter Regions Genetic lcsh:Science Multidisciplinary Genetically Modified Organisms Eukaryota Vector Construction Plants Experimental Organism Systems Engineering and Technology Cellular Structures and Organelles Genetic Engineering Research Article Biotechnology DNA Plant Imaging Techniques Transgene Arabidopsis Thaliana Genetic Vectors Bioengineering Computational biology Brassica Biology DNA construction 03 medical and health sciences Model Organisms Plant and Algal Models Fluorescence Imaging Gene Expression and Vector Techniques Peroxisomes Marchantia Cloning Molecular Biology Assays and Analysis Techniques DNA manipulations DNA fragment ligation lcsh:R Organisms Biology and Life Sciences Cell Biology Vector Cloning biology.organism_classification Artificial Gene Fusion Research and analysis methods 030104 developmental biology Molecular biology techniques Animal Studies Plant Biotechnology lcsh:Q 010606 plant biology & botany |
| Zdroj: | PLoS ONE, Vol 13, Iss 10, p e0204964 (2018) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | The liverwort Marchantia polymorpha is an emerging model species for basal lineage plant research. In this study, two Gateway cloning-compatible binary vector series, R4pMpGWB and R4L1pMpGWB, were generated to facilitate production of transgenic M. polymorpha. The R4pMpGWB series allows tripartite recombination of any promoter and any coding sequence with a specific reporter or tag. Reporters/tags for the R4pMpGWB series are GUS, ELuc(PEST), FLAG, 3×HA, 4×Myc, mRFP1, Citrine, mCitrine, ER-targeted mCitrine and nucleus-targeted mCitrine. The R4L1pMpGWB series is suitable for promoter analysis. R4L1pMpGWB vector structure is the same as that of R4pMpGWB vectors, except that the attR2 site is replaced with attL1, enabling bipartite recombination of any promoter with a reporter or tag. Reporters/tags for the R4L1pMpGWB series are GUS, G3GFP-GUS, LUC, ELuc(PEST), Citrine, mCitrine, ER-targeted mCitrine and mCitrine-NLS. Both vector series were functional in M. polymorpha cells. These vectors will facilitate the design and assembly of plasmid constructs and generation of transgenic M. polymorpha. |
| Databáze: | OpenAIRE |
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