Isolation of sperm storage tubules from the uterovaginal junction mucosa of the turkey
| Autor: | M. R. Bakst, J-P. Brillard, L. M. King, Annie M. Donoghue |
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| Rok vydání: | 1999 |
| Předmět: |
Male
Turkeys endocrine system Lumen (anatomy) Oviducts Andrology 03 medical and health sciences fluids and secretions 0302 clinical medicine parasitic diseases medicine Animals 030304 developmental biology 0303 health sciences Long axis Mucous Membrane 030219 obstetrics & reproductive medicine Enzymatic digestion Chemistry Uterus fungi Large white Motile sperm General Medicine Spermatozoa Sperm Epithelium medicine.anatomical_structure Tubule Vagina Female Animal Science and Zoology hormones hormone substitutes and hormone antagonists |
| Zdroj: | Poultry Science. 78:1044-1047 |
| ISSN: | 0032-5791 |
| DOI: | 10.1093/ps/78.7.1044 |
| Popis: | This study was performed to determine whether intact sperm storage tubules (SST) could be successfully isolated from the uterovaginal junction (UVJ) mucosa of the turkey. Large White BUTA hens were inseminated and euthanatized 24 to 48 h later. Oviducts were excised, UVJ tissue removed, and SST were procured by enzymatic digestion. Recovered SST were intact and contained motile sperm. The sperm were oriented with their acrosomes pointed towards the distal end of the SST, and their long axes in parallel with the long axis of the tubule's lumen. This method for the isolation of intact SST can be readily applied for in vitro culture studies as well as for the extraction of DNA and RNA from the SST epithelium. |
| Databáze: | OpenAIRE |
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