Additional file 1 of RNF31 represses cell progression and immune evasion via YAP/PD-L1 suppression in triple negative breast Cancer

Autor: Yang, Huijie, Xue, Min, Su, Peng, Zhou, Yan, Li, Xin, Li, Zhongbo, Xia, Yan, Zhang, Chenmiao, Fu, Mingxi, Zheng, Xiuxia, Luo, Guosheng, Wei, Tian, Wang, Xinxing, Ding, Yinlu, Zhu, Jian, Zhuang, Ting
Rok vydání: 2023
DOI: 10.6084/m9.figshare.22606181.v1
Popis: Additional file 1 Supplementary Fig. 1 A Western blot detecting of RNF31 expression in HS578T cell exposed to indicated methods. B-C Transwell assay (left panel) of HS578T cells. Right panel shows quantification of transwell assay results. Scale bar 100 μm. D-E FACS analysis (left panel) was performed on the HS578T cell to detect the proportion of CD44 + CD24-cells. F-G FACS analysis (left panel) was performed on the HS578T cell to detect the proportion of apoptotic cells. The cells were incubated with PI and Annexin V. Right panel shows quantification of apoptosis proportion. H-I Wound healing assay (left panel) of HS578T cell migration capability following transfected with indicated treatment. J-K Representative images (left panel) of EdU assays in HS578T cell transfected with indicated treatment. EdU-positive cells, red; cell nuclei, blue. Right panel shows quantification of Edu results. Scale bar 100 μm.
Databáze: OpenAIRE